0
0
0
0
Smart Citations
0
0
0
0
Citing PublicationsSupportingMentioningContrasting
View Citations

See how this article has been cited at scite.ai

scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

Sodium nitroprusside induces cell death and cytoskeleton degradation in adult rat cardiomyocytes in vitro: implications for anthracycline-induced cardiotoxicity

Authors

Sodium nitroprusside (SNP) is used clinically as a rapid-acting vasodilator and in experimental models as donor of nitric oxide (NO). High concentrations of NO have been reported to induce cardiotoxic effects including apoptosis by the formation of reactive oxygen species. We have therefore investigated effects of SNP on the myofibrillar cytoskeleton, contractility and cell death in long-term cultured adult rat cardiomyocytes at different time points after treatment. Our results show, that SNP treatment at first results in a gradual increase of cytoskeleton degradation marked by the loss of actin labeling and fragmentation of sarcomeric structure, followed by the appearance of TUNEL-positive nuclei. Already lower doses of SNP decreased contractility of cardiomyocytes paced at 2 Hz without changes of intracellular calcium concentration. Ultrastructural analysis of the cultured cells demonstrated mitochondrial changes and disintegration of sarcomeric alignment. These adverse effects of SNP in cardiomyocytes were reminiscent of anthracycline-induced cardiotoxicity, which also involves a dysregulation of NO with the consequence of myofibrillar degradation and ultimately cell death. An inhibition of the pathways leading to the generation of reactive NO products, or their neutralization, may be of significant therapeutic benefit for both SNP and anthracycline-induced cardiotoxicity.

Downloads

Publication Facts

Metric
This article
Other articles
Peer reviewers 
2
2.4

Reviewer profiles  N/A

Author statements

Author statements
This article
Other articles
Data availability 
N/A
16%
External funding 
N/A
32%
Competing interests 
Yes
11%
Metric
This journal
Other journals
Articles accepted 
58%
33%
Days to publication 
292
145

Indexed in

Editor & editorial board
profiles
Academic society 
N/A

PFL

1 2 3 4 5
Not useful Very useful

Citations

Supporting Agencies

This work was supported by Swiss National Science Foundation grant 3100A0-120664 to C. Zuppinger and a grant of the Gebert-Ruef foundation (GRS 038/01) to J.C. Perriard
M. Chiusa, Bern University Hospital

University Hospital Bern, Department Heart and Vessels

Scientist

F. Timolati, Bern University Hosptial

University Hospital Bern, Department Heart and Vessels

Scientist

J.C. Perriard, Federal Institute of Technology, ETHZ, Zürich
ETHZ, professor emeritus
T.M. Suter, Bern University Hospital

University Hospital Bern, Department Heart and Vessels

professor of cardiology

C. Zuppinger, Bern University Hospital

University Hospital Bern, Department Heart and Vessels

Senior Scientist

How to Cite

Chiusa, M., Timolati, F., Perriard, J., Suter, T., & Zuppinger, C. (2012). Sodium nitroprusside induces cell death and cytoskeleton degradation in adult rat cardiomyocytes in vitro: implications for anthracycline-induced cardiotoxicity. European Journal of Histochemistry, 56(2), e15. https://doi.org/10.4081/ejh.2012.15

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.