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3-hydroxi-anthranilic acid is early expressed in stroke

A. Mangas, J. Yajeya, N. González, I. Ruiz, M. Geffard, R. Coveñas
  • A. Mangas
    Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT) - University of Salamanca, France | amangas@gemacbio.com
  • J. Yajeya
    University of Salamanca, Department of Physiology, Spain
  • N. González
    Institut pour le Développement de la Recherche en Pathologie Humaine et Thérapeutique (IDRPHT), France
  • I. Ruiz
    Gemacbio, France
  • M. Geffard
    Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT), France
  • R. Coveñas
    University of Salamanca, Spain

Abstract

Using an immunohistochemical technique, we have studied the distribution of 3-OH-anthranilic acid (3-HAA) in the rat brain. Our study was carried out in control animals and in rats in which a stroke model (single transient middle cerebral artery occlusion) was performed. A monoclonal antibody directed against 3-HAA was also developed. 3-HAA was exclusively observed in the infarcted regions (ipsilateral striatum/cerebral cortex), 2, 5 and 21 days after the induction of stroke. In control rats and in the contralateral side of the stroke animals, no immunoreactivity for 3-HAA was visualized. Under pathological conditions (from early phases of stroke), we reported for the first time the presence of 3-HAA in the mammalian brain. By double immunohistochemistry, the coexistence of 3-HAA and GFAP was observed in astrocytes. The distribution of 3-HAA matched perfectly with the infarcted regions. Our findings suggest that, in stroke, 3-HAA could be involved in the tissue damage observed in the infarcted regions, since it is well known that 3-HAA exerts cytotoxic effects.

Keywords

Astrocyte; ischemia; glia; immunohistochemistry; monoclonal antibody; neurotoxicity; 3-OH-anthranilic acid.

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Submitted: 2016-08-01 14:40:14
Published: 2016-12-02 09:30:36
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Copyright (c) 2016 Arturo Mangas, Javier Yajeya, Noelia González, Isabel Ruiz, Michel Geffard, Rafael Coveñas

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