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To the ring-shaped nucleolus seen by microscopy using human lymphocytes of blood donors and chronic lymphocytic leukemia patients

Vol. 68 No. 3 (2024)
Submitted: 5 June 2024
Accepted: 22 June 2024
Published: 19 August 2024
Ring-shaped nucleolus, human lymphocytes
Abstract Views: 708
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Authors

Karel Smetana
Karel.Smetana@uhkt.cz
https://orcid.org/0009-0007-2316-1762
Institute of Haematology and Blood Transfusion, Prague, Czechia.
Dana Mikulenková
Institute of Hematology and Blood Transfusion, Prague, Czechia.
Josef Karban
https://orcid.org/0000-0003-4199-6476
1st Hematology and Oncology Clinic of the 1st Medical Faculty of the Charles University and General Medical Hospital, Prague, Czechia.
Marek Trněný
https://orcid.org/0000-0002-6952-6073
1st Hematology and Oncology Clinic of the 1st Medical Faculty of the Charles University and General Medical Hospital, Prague, Czechia.

The present study was undertaken to provide more information on the peripheral RNA containing ring of ringshaped nucleoli (RSNo). Human lymphocytes of blood donors and patients suffering from B chronic lymphocytic leukemia mostly characterized by RSNo represented very convenient cell models for such study. According to the light microscopy the peripheral RNA ring possessed several highly condensed foci. Such regions represented accumulated dense RNA fibrillar components (DFCs) seen by the electron microscopy. In contrary, the incidence of dense granular RNA-containing components (GCs) in surrounding portions of the RNA ring was small. Thus, the structural and morphological organization of the peripheral RNA ring of RSNo apparently reflects sites of micro-segregated foci of DFCs and a small incidence of GCs. That structural organization of the peripheral RNA ring of RSNo appeared to be a prerequisite for further regressive nucleolar changes resulting in the development of micronucleoli in terminal lymphocytes.

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Supporting Agencies

Institute of Hematology and Blood Transfusion - Ministry of Health of the Czech Republic, Hermansky Foundation of Hematological Oncology

How to Cite

Smetana, K., Mikulenková, D., Karban, J., & Trněný, M. (2024). To the ring-shaped nucleolus seen by microscopy using human lymphocytes of blood donors and chronic lymphocytic leukemia patients. European Journal of Histochemistry, 68(3). https://doi.org/10.4081/ejh.2024.4075
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