https://doi.org/10.4081/ejh.2024.4075

To the ring-shaped nucleolus seen by microscopy using human lymphocytes of blood donors and chronic lymphocytic leukemia patients

Vol. 68 No. 3 (2024)
Submitted: 5 June 2024
Accepted: 22 June 2024
Published: 19 August 2024
Ring-shaped nucleolus, human lymphocytes
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Authors

Karel Smetana
Karel.Smetana@uhkt.cz
https://orcid.org/0009-0007-2316-1762
Institute of Haematology and Blood Transfusion, Prague, Czechia.
Dana Mikulenková
Institute of Hematology and Blood Transfusion, Prague, Czechia.
Josef Karban
https://orcid.org/0000-0003-4199-6476
1st Hematology and Oncology Clinic of the 1st Medical Faculty of the Charles University and General Medical Hospital, Prague, Czechia.
Marek Trněný
https://orcid.org/0000-0002-6952-6073
1st Hematology and Oncology Clinic of the 1st Medical Faculty of the Charles University and General Medical Hospital, Prague, Czechia.

The present study was undertaken to provide more information on the peripheral RNA containing ring of ringshaped nucleoli (RSNo). Human lymphocytes of blood donors and patients suffering from B chronic lymphocytic leukemia mostly characterized by RSNo represented very convenient cell models for such study. According to the light microscopy the peripheral RNA ring possessed several highly condensed foci. Such regions represented accumulated dense RNA fibrillar components (DFCs) seen by the electron microscopy. In contrary, the incidence of dense granular RNA-containing components (GCs) in surrounding portions of the RNA ring was small. Thus, the structural and morphological organization of the peripheral RNA ring of RSNo apparently reflects sites of micro-segregated foci of DFCs and a small incidence of GCs. That structural organization of the peripheral RNA ring of RSNo appeared to be a prerequisite for further regressive nucleolar changes resulting in the development of micronucleoli in terminal lymphocytes.

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Citations

Crossref
Scopus
Google Scholar
Europe PMC
Bernhard W, Granboulan N. The fine structure of the cancer cell nucleus. Exp Cell Res 1963;9:S19-53. DOI: https://doi.org/10.1016/0014-4827(63)90243-X
Busch H, Smetana K. The nucleolus. New York, Academic Press, 1970.
Smetana K, Busch H. The nucleolus and nucleolar DNA. In: H. Busch, editor. The cell nucleus. New York: Academic Press; 1974. p. 75-148. DOI: https://doi.org/10.1016/B978-0-12-147601-4.50010-5
Hernanadez-Verdun D Structural organization of the nucleolus in mammalian cell. Meth Arch Exp Pathol 1986;12:26-62.
Smetana K, Jirásková I, Sedláčková M, Dvořák R, Špátová M, Hozák P. Preferential silver reaction of nucleolar regions adjacent to fibrillar centers in ring shaped nucleoli of leukemic lymphocytes. Acta Histochem 1998;100:257-70. DOI: https://doi.org/10.1016/S0065-1281(98)80012-3
Smetana K, Jirásková I, Perlaky L, Busch H. The silver reaction of nucleolar proteins in the main structural compartments of ring-shaped nucleoli in smear preparations. Acta Histochem 1999;101:167-83. DOI: https://doi.org/10.1016/S0065-1281(99)80016-6
Smetana K. The nucleolus through the years. J Appl Biomed 2011;9:119-27. DOI: https://doi.org/10.2478/v10136-011-0010-7
Smetana K, Lejnar J, Šálková J. Studies on nucleoli in rosetting T and B lymphocytes of the human peripheral blood. Folia Haematol 1980;107:720-7.
Smetana K, Lejnar J, Potměšil M. A further contribution to the demonstration of RNA and nucleoli in smear preparations. Folia Haematol 1969;91:381-4.
Ochs RL. Methods used to study structure and function of the nucleolus. Methods Cell Biol 1998:53:303-21. DOI: https://doi.org/10.1016/S0091-679X(08)60884-5
Smetana K. Electron microscopy of lymphocytes. In: H. Busch, editor. Methods in cancer research. New York: Academic Press; 1970. p. 455-78.
Hoffbrand AV, Pettit JE. Clinical haematology (Sandoz Atlas). London: Gower Medical Publishing; 1988.
Matutes E, Polliack. A Morphological and immunophenotypic features of chronic lymphocytic leukemia. Rev Clin Exp Hematol 2000;4:22-47. DOI: https://doi.org/10.1046/j.1468-0734.2000.00002.x
Karasaki S. Electron microscopic examination of the sites of nuclear RNA synthesis during amphibian embryogenesis. J Cell Biol 1965;26:937-58. DOI: https://doi.org/10.1083/jcb.26.3.937
Granboulan N, Granboulan P. [Cytochimie ultrastructurale du nucléole: II. Étude des sites de synthèse du RNA dans le nucléole et le noyau].[Article in French]. Exp Cell Res 1965;38:604-19. DOI: https://doi.org/10.1016/0014-4827(65)90384-8
Fakan S. Structural support for RNA synthesis in the cell nucleus. Methods Achiev Exp Pathol 1986;12:105-40.
Hozák P, Cook PR, Schofer, C, Mosgoller W, Wachtler F. Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells. J Cell Sci 1994;107:639-48. DOI: https://doi.org/10.1242/jcs.107.2.639
Trentani A, Testillano PS, Risueño MC, Biggiogera M. Visualization of transcription sites at the electron microscope. Eur J Histochem 2003;47:195-200. DOI: https://doi.org/10.4081/827
Raška I, Shaw PJ, Cmarko D. Structure and function of the nucleolus in the spotlight. Curr Opin Cell Biol 2006;18:325-34. DOI: https://doi.org/10.1016/j.ceb.2006.04.008
Penzo M, Montanaro L, Treré D, Derenzini M. The ribosome biogenesis - cancer connection. Cells 2019;8:55. DOI: https://doi.org/10.3390/cells8010055
Vandelaer M, Thiry M, Goessens G. Ultrastructural distribution of DNA within the ring-shaped nucleolus of human resting T lymphocytes. Exp Cell Res 1993;205:430-2. DOI: https://doi.org/10.1006/excr.1993.1110
Rubin AD. Defective control of ribosomal RNA processing in stimulated leukemic lymphocytes. J Clin Invest 1971;50:2485-97. DOI: https://doi.org/10.1172/JCI106749
Smetana K. Nucleoli in maturing blood cells. In: S. Roath, editor. Topical reviews in haematology. Bristol: Wright Ltd.; 1980. p. 115-37.

Supporting Agencies

Institute of Hematology and Blood Transfusion - Ministry of Health of the Czech Republic, Hermansky Foundation of Hematological Oncology

How to Cite

Smetana, K., Mikulenková, D., Karban, J., & Trněný, M. (2024). To the ring-shaped nucleolus seen by microscopy using human lymphocytes of blood donors and chronic lymphocytic leukemia patients. European Journal of Histochemistry, 68(3). https://doi.org/10.4081/ejh.2024.4075
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