Expression pattern of thymosin beta 4 in the adult human liver

S. Nemolato,¹ P. Van Eyken,² T. Cabras,³ F. Cau,¹ M.U. Fanari,¹ A. Locci,¹ D. Fanni,¹ C. Gerosa,¹ I. Messana,³ M. Castagnola,⁴ G. Faa¹

¹Dipartimento di Citomorfologia, Divisione di Anatomia Patologica, Università di Cagliari, Italy; ²Department of Pathology, K.U. Leuven, Belgium; ³Dipartimento di Scienze Applicate ai Biosistemi, Università di Cagliari, Italy; ⁴Dipartimento di Biochimica Clinica, Universita Cattolica di Roma, Italy



Correspondence: Dr. Sonia Nemolato, Dipartimento di Citomorfolgia, Sezione di Anatomia Patologica, AOU San Giovanni di Dio, via Ospedale 54, 09124 Cagliari, Italy. Tel. +39.070.6092370 - Fax: +39.070.657882. E-mail: sonianemolato@libero.it

Key words: β-Thymosins, Tβ4, human liver.

Acknowledgments: the authors would express their deep gratitude to Prof. Matteo Fraschini (University of Cagliari) for critical suggestions. They also want to thank Ms. Sandra Serra, Simonetta Paderi and Rossana Zedda for technical assistance and Mr. Ignazio Ferru for administrative support. This study was supported by Fondazione Banco di Sardegna.

Contributions: SN, PVE, IM, MC, GF research design, manuscript writing, revising and final approval; SN, PVE, TC, DF, CG research performing; FC, MUF, AL contributing to reagents/analytic tools; SN, PVE, TC, DF, CG, IM, MC, GF, data analysis.

Conflict of interest: the authors report no conflicts of interest.

Received for publication: 26 February 2011.
Accepted for publication: 6 June 2011.

This work is licensed under a Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0).

©Copyright S. Nemolato et al., 2011
Licensee PAGEPress, Italy
European Journal of Histochemistry 2011; 55:e25
doi:10.4081/ejh.2011.e25





Introduction


Thymosin beta 4 (Tβ4) is a member of thymosins,1 a versatile family of small peptides expressed in most mammalian cells.2 It shows a high intracellular concentration, in cells able to rapidly increase their motility, like polymorphonuclear leukocytes, macrophages3,4 and mast cells.5 There exist about 15 highly homologous β-thymosin variants, containing 40 to 44 amino acid residues, that are found not only inside of cells but also in extracellular fluids, such as blood serum,6 saliva,7 wound fluid,8 and lacrimal secretion.9 Among thymosin peptides, Tβ4 is considered the most abundant in mammalian tissues: its activity has been mainly correlated with the regulation of actin polymerization in living cells.10 Tb4 is thought to be also involved in many critical biological processes including angiogenesis,11 wound healing,12 inflammatory response13 and cell migration.14 Similarly, Tβ4 activity was implicated in differentiation of hematopoietic cells,15 in carcinogenesis,16 in apoptosis,17 and in blood coagulation.18
Recently, many authors have proposed the Tβ4 involvement in the process of determining fibrosis in different organs. In mice with ureteral obstruction, Tβ4 has been shown to have profibrotic effects, while its metabolite Ac-SDKP showed anti-fibrotic actions.19 In human hepatic stellate cells in culture, Tβ4 showed antifibrogenic effects, mediated by upregulation of the expression of hepatocyte grow factor (HGF) and by downregulation of the expression of platelet-derived growth factor-β (PDGF-β) receptor.20 A recent study from our group carried out on the human newborn liver did not reveal any significant reactivity for Tβ4 in the vast majority of liver cells during gestation and at birth.21
On the basis of these data, and given the previously reported ability of thymosins to change their expression pattern at different ages, it seemed of some interest to investigate by immunohistochemistry the Tβ4 expression pattern in the adult liver, in order to shed light on the role played by liver cells in the synthesis of this peptide.

Materials and Methods

The study included archived paraffin-embedded liver sections obtained from 35 subjects. The cohort included 11 needle liver biopsies, performed on different clinical settings, in which no sign of liver pathology was detected at the histological examination, and 24 liver samples obtained in autopsy patients who died for different extrahepatic aetiologies, in which careful macroscopic and histological examination of liver sections did not evidence any significant pathological change. The age of subjects (24 males and 11 females) ranged from 29 up to 77 years. Clinical data at the basis of the decision of performing liver biopsies are reported in Table 1; the cause of death of the 24 autopsy cases is reported in Table 2. All liver samples were routinely formalin-fixed and paraffin-embedded.
Staining of formalin-fixed, paraffin-embedded 5 μm sections for Tβ4 was performed essentially as previously described.5 Briefly, slides were deparaffinized, rehydrated, and endogenous peroxidase activity was quenced (30 min) by 0.3% hydrogen peroxide in methanol. Slides were then subjected to antigen retrival by boiling (20 min) in 10 mM citrate buffer, pH 6. Slides were incubated with 10% normal goat serum in phosphate buffered saline (PBS) for 60 min to block non-specific binding, followed by incubation (20 h, 4°C) with polyclonal anti-thymosin beta 4 antibody (Bachem-Peninsula Lab, San Carlos, CA, USA) diluted 1:100 in blocking solution. Slides were extensively washed with PBS containing 0.01% Triton X-100 and incubated with a secondary reagent (Envision kit) according to the manufacturer’s (Dako, Glostrup, Denmark) instructions. After additional washes, color was developed using AEC reagent (Dako), sections were counterstained with hematoxylin and mounted. Immunostained specimens were examined by two pathologists (GF, SN). A semiquantitative grading system for the evaluation of Tβ4 immunoreactivity has been applied: + = focal weak staining; ++ = diffuse weak staining; +++ = diffuse strong staining. Zone 3 (periterminal hepatocytes) and zone 1 (periportal hepatocytes) were independently analyzed for Tβ4 immunoreactivity.

Table 1. clincal and immunohistochemical data of 11 liver needle biopsies characterized at histology by absent or minimal changes. + = weak and focal; ++ = weak and diffuse; +++ = strong and diffuse.

Table 2. Clinical and immunohistochemical data regarding 25 liver samples obtained at autopsy, all characterized at histology by absent or minimal changes. + = weak and focal; ++ = weak and diffuse; +++ = strong and diffuse.

Results

Tβ4 reactivity was detected in all normal liver biopsies of adult subjects studied. No significant difference was observed between bioptic and autoptic liver examined. No relationship was found between the degree of Tβ4 immunostaining and age or sex of the subjects. Immunoreactivity for the peptide was mainly localized in the cytoplasm of hepatocytes, appearing as large granules different in shape and size. At low power, in the vast majority of cases, immunoreactivity for Tβ4 was not homogeneously diffuse to the entire parenchyma: it was mainly stored in periterminal hepatocytes (zone 3 of the acinus) while a minor degree of reactivity was detected in periportal hepatocytes (zone 1) (Figure 1). We often detected a zonation of Tβ4 expression, characterized by a progressive increase in the degree of immunostaining from the periportal to the periterminal areas, intermediate levels of Tβ4 immunostaining being observed in the hepatocytes of acinar zone 2. In some cases (see Table 1 and Table 2), the periportal hepatocytes did not show any reactivity for the peptide: in these cases, only a mild reactivity for the peptide was found in zone 3 liver cells, indicating a strict correlation between Tβ4 storage in the different acinar zones. The degree of reactivity changed from one case to the next. In some cases only the periterminal hepatocytes were reactive for the peptide (Figure 2), while in other cases even periportal hepatocytes were immunostained (Figure 3). Tβ4-reactive intracytoplasmic granules appeared mainly localized at the biliary pole of hepatocytes (Figure 4). In cases with a strong immunostaining, even perinuclear areas and the sinusoidal pole of hepatocytes appeared interested by Tβ4 overload (Figure 5). Even the size of Tβ4 granules changed from one case to the next, the largest immunoreactive granules constantly being observed in cases characterized by the highest levels of reactivity for the peptide (Figure 5). The vast majority of stored peptide was constantly found inside the cytoplasm of the hepatocytes, in the absence of significant Tβ4 staining in Kupffer cells and in Ito cells.

Figure 1. Tb4 immunoreactivity is localized in the periterminal hepatocytes. A minor reactivity for the peptide is detected in the periportal hepatocytes. Scale bar: 250 μm.

Figure 2. A strong Tb4-imunoreactivity is observed in the periterminal hepatocytes. Scale bar: 400 μm.

Figure 3. A lower Tb4-immunoreactivity is detected in periportal hepatocytes. Scale bar: 400 μm.

Figure 4. Tb4-reactive intracytoplasmic granules are localized at the biliary pole of hepatocytes. Scale bar: 400 μm.

Figure 5. Tb4-reactive granules with a perinuclear distribution in the hepatocytes are detected. Tb4 immunoreactivity at the sinusoidal pole of the cytoplasm of the hepatocytes is observed. Scale bar: 400 μm.

Discussion

For some decades from the discovery of Tβ4 in the calf thymus in 1965,22 researchers have looked at its distribution in different organs and tissues and asked, what does it mean? To date, the majority of attempts to elucidate the mechanism of action of Tβ4 in living organisms has come from the perspective of physiologists, biochemists and biologists and, possibly, have lacked an understanding of the role played by this peptide in human health and disease.
To this date, at the best of our knowledge, only one study from our group has investigated Tβ4 expression in the human liver in newborns and during foetal development.21 In that study, Tβ4 was not detected in liver cells, in hepatocytes, in ductal cells and in oval cells of the ductal plate, whereas low levels of expression were occasionally detected in isolated cells inside the loose connective tissue of immature portal tracts. These data were in favour of a minor role of human liver in Tβ4 metabolism. On the contrary, the current work evidences striking differences between the adult human liver and the newborn liver as for Tβ4 immunoreactivity. The two conditions, the newborn and the adult, appear to represent the extremes of a spectrum: complete absence of the peptide in liver cells at birth, and a strong diffuse accumulation in the hepatocytes in some adults.
It is unclear why this difference in the amount of Tβ4 exists between adults and newborns, but our findings confirm previous reports on the ability of Tβ4, as well of other beta-thymosins, to change its expression during life, both quantitatively and qualitatively. In human salivary glands, we first showed a decrease in the degree of Tβ4 expression from the foetus and the newborn to adults.23 Moreover, we also showed a modification of the protein expression pattern, which was granular and mainly localized inside the acinar lumen in newborns, while it appeared homogeneously diffuse to the entire cytoplasm in adults.23 The granular reactivity was interpreted as a secretory pattern, explaining the previously reported presence of large amounts of Tβ4 in saliva of newborns;7 the weak homogeneous staining of the entire cytoplasm was interpreted as a structural pattern, putatively related to the G-actin binding activity of Tβ4.2,20 The present study adds strenght to a marked variability in the expression and function of Tβ4 in different organs, showing a completely opposite modification in the peptide expression in the liver, characterized by the absence of Tβ4 at birth and its impressive synthesis in the adult.
The high intracellular concentration of Tβ4 observed in normal hepatocytes deserves some consideration on the not well known activity of this versatile peptide. The main intracellular action of β-thymosins in resting cells has generally been considered their ability to sequester monomeric actin, inhibiting its ability to polymerize and therefore removing it from the cycling actin pool.24 During cell activation for cellular activities like locomotion, actin monomers are rapidly desequestrated in order to achieve the formation of actin filaments.2 As a consequence, the intracellular concentration of Tβ4 has been found to be particularly high in cells that rapidly respond to external signals by profound shape changes like platelets25,26 or by increased motility like polymorphonuclear cells,27 mast cells5 and macrophages.28,29 The observation in this study of large amounts of Tβ4 in the hepatocytes, i.e. in cells which do not rapidly change their shape or their motility, clearly supports the hypothesis that Tβ4 is not a simple actin monomer binding protein.3 The massive hepatic Tβ4 expression described in this study in some subjects, adds hepatocytes to the list of human cells able to sinthesize large amounts of the peptide in adulthood. The serum concentration of free Tβ4 has been estimated to range from 0.45 up to 1.1 μg/L,6 which is very high when compared to the concentration of insulin or glucagon,2 but comparable to the adenosin concentration.30 Such an high serum level of Tβ4 in the steady state can not be explained by its release by platelets during blood coagulation24 or by leukocytes and monocytes during inflammation.31 On the contrary, the present work induces to hypothesize that the high serum content of Tβ4 could, at least in part, originate by hepatocytes. Further studies correlating the hepatic Tβ4 expression pattern with serum levels of the peptide will clarify the role of liver cells in plasma Tβ4 levels.
The preferential concentration of Tβ4 in the hepatocytes of zone 3 of the liver acinus also deserves some consideration. The descending gradient from the terminal vein towards the periportal areas indicate that the peptide is mainly synthesized in zone 3 hepatocytes and, only when major quantities of Tβ4 are needed, even zone 2 and zone 1 hepatocytes are recruited for its additional synthesis. A similar zonation pattern, with high expression in the downstream perivenous region, has ben described for many liver enzymes, including Cytochrome P-45032 and glutamine synthetase.33 By comparison with our knowledge on the acinar gradients of liver proteins, now including Tβ4, much less is known about how these gradients are formed, modified and maintained. Different concentrations of oxygen and of humoral factors have been proposed to may create a gradient-type zonation pattern.32 In recent years, a fundamental role in mechanisms involved in patterning of liver parenchyma has been attributed to morphogens, including Wnt, β-catenin, and adenomatous polyposis coli gene.33 As for the gradient-type zonation observed in this study for Tβ4, our data show a marked interindividual variability, not only in the degree of expression but even in the zonation pattern. This finding suggests that the intensity of Tβ4 synthesis and the slope of the gradient can vary. Diurnal variations in the degree and in spatial expression have been reported for other liver enzymes, including CYP7.34 The low levels of Tβ4 expression in all liver biopsies from subjects positive for HBV or HCV markers (Table 1) induces to hypothesize a possible role for hepatitis-related viruses in down-regulating Tβ4 synthesis in infected liver cells.
In conclusion, interesting data emerge from the analysis of Tβ4 expression in liver cells, clearly indicating Tβ4 as one of the proteins synthesized by hepatocytes in normal conditions. Identifying the intimate role played by this peptide intracellularly and extracellularly, in physiology and in liver diseases, is a major challenge for future research focusing on Tβ4 expression in the human liver in health and disease.

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