https://doi.org/10.4081/1685

Simultaneous demonstration of acid phosphatase and glucose-6-phosphate dehydrogenase in mouse hepatocytes. A novel electron-microscopic dual staining enzyme-cytochemistry

Vol. 46 No. 3 (2002)
Submitted: 23 January 2010
Accepted: 23 January 2010
Published: 23 January 2010
Abstract Views: 645
PDF: 528
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Authors

S Matsubara
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PAGEPress Office, Pavia, Italy.
Acid phosphatase (ACPase) and glucose-6-phosphate dehydrogenase (G6PD) play important roles in cell biology/disease pathophysiology in various organs including the liver. The purpose of the present report is to introduce a new enzymecytochemical method to simultaneously demonstrate the subcellular localization of ACPase and G6PD within the same hepatocyte in the mouse liver. The ultrastructural localization of ACPase and G6PD were demonstrated, with concomitant use of the cerium method and the copper-ferrocyanide method, respectively. ACPase labelings were localized in the lysosomes, and G6PD labelings were visible in the cytoplasm and on the cytosolic side of the endoplasmic reticulum of the hepatocyte. This novel double staining procedure may be a useful histochemical tool for the study of liver functions in both physiological and pathological conditions.

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How to Cite

Matsubara, S. (2010). Simultaneous demonstration of acid phosphatase and glucose-6-phosphate dehydrogenase in mouse hepatocytes. A novel electron-microscopic dual staining enzyme-cytochemistry. European Journal of Histochemistry, 46(3), 237–42. https://doi.org/10.4081/1685

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