Drug-DNA interaction protocols

In a paper published by Jurgen Drews on the march 17, 2000 issue of Science a figure reporting molecular targets of drug therapy, classified according to biochemical criteria, showed that only 2% of drugs were directed to DNA. Presently, the number of DNA targeting molecules, primarily for Cancer treatment, is increasing along with the number of applications in medicine. The design of such molecules relies on the quantitative assessment of the binding strength of small molecules to DNA. For this purpose various techniques suitable to work in the subnanomolar range have been developed......