G-quadruplex DNA - Methods and protocols

Thanks to the end of several genome sequencing projects it has become a reality the notion that mammal genomes (notably mouse and human) harbor hundreds of thousands of Guanine-quartets which have the potential to interact to form Guanine-quadruplexes, that is to say, non-standard DNA conformation of stabilized planar arrangement of four guanine bases. Then, in very recent years, it becomes clear enought that G-quadruplexes have general functions in Biology, not only at the telomeric ends (where the canonic sequence of TTAGGG is particularly prone to form G-quadruplexes), such as playing roles in processes as different as gene expression, DNA recombination, DNA transcription, mRNA maturation and translation......