The biocompatibility of porous vs non-porous bone cements: a new methodological approach

  • C. Dall'Oca | carlo.dalloca@univr.it University of Verona, Italy.
  • T. Maluta University of Verona, Italy.
  • F. Cavani University of Modena, Italy.
  • G.P. Morbioli University of Verona, Italy.
  • P. Bernardi University of Verona, Italy.
  • A. Sbarbati University of Verona, Italy.
  • D. Degl'Innocenti University of Verona, Italy.
  • B. Magnan University of Verona, Italy.

Abstract

Composite cements have been shown to be biocompatible, bioactive, with good mechanical properties and capability to bind to the bone. Despite these interesting characteristic, in vivo studies on animal models are still incomplete and ultrastructural data are lacking. The acquisition of new ultrastructural data is hampered by uncertainties in the methods of preparation of histological samples due to the use of resins that melt methacrylate present in bone cement composition. A new porous acrylic cement composed of polymethylmetacrylate (PMMA) and β-tricalciumphosphate (β-TCP) was developed and tested on an animal model. The cement was implanted in femurs of 8 New Zealand White rabbits, which were observed for 8 weeks before their sacrifice. Histological samples were prepared with an infiltration process of LR white resin and then the specimens were studied by X-rays, histology and scanning electron microscopy (SEM). As a control, an acrylic standard cement, commonly used in clinical procedures, was chosen. Radiographic ultrastructural and histological exams have allowed finding an excellent biocompatibility of the new porous cement. The high degree of osteointegration was demonstrated by growth of neo-created bone tissue inside the cement sample. Local or systemic toxicity signs were not detected. The present work shows that the proposed procedure for the evaluation of biocompatibility, based on the use of LR white resin allows to make a thorough and objective assessment of the biocompatibility of porous and non-porous bone cements.

Downloads

Download data is not yet available.

Author Biographies

C. Dall'Oca, University of Verona

Department of Surgery, Orthopaedic and Traumatology Clinic

T. Maluta, University of Verona
Department of Surgery, Orthopaedic and Traumatology Clinic
F. Cavani, University of Modena

Department of Anatomy and Histology

G.P. Morbioli, University of Verona

Interdepartmental Center of Scientific Research on Laboratory Animals

P. Bernardi, University of Verona

Department of Neurological, Neuropsychological, Morphological and Motor Sciences

A. Sbarbati, University of Verona
Department of Neurological, Neuropsychological, Morphological and Motor Sciences
D. Degl'Innocenti, University of Verona
Research grant of Department of surgery, pHD in Magnetic Resonance Imaging
B. Magnan, University of Verona
Department of Surgery, Orthopaedic and Traumatology Clinic
Published
2014-06-23
Section
Original Papers
Keywords:
Calcium phosphate cement, osteointegration, biocompatibility, osteoconduction, porosity.
Statistics
Abstract views: 1131

PDF: 285
HTML: 584
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Dall’Oca, C., Maluta, T., Cavani, F., Morbioli, G., Bernardi, P., Sbarbati, A., Degl’Innocenti, D., & Magnan, B. (2014). The biocompatibility of porous vs non-porous bone cements: a new methodological approach. European Journal of Histochemistry, 58(2). https://doi.org/10.4081/ejh.2014.2255