Immunohistochemical analysis of matrix metalloproteinase-13 in human caries dentin

  • C. Loreto | carla.loreto@unict.it University of Catania, Italy.
  • C. Galanti University of Catania, Italy.
  • G. Musumeci University of Catania, Italy.
  • M. C. Rusu "Carol Davila" University of Medicine and Pharmacy, Romania.
  • R. Leonardi University of Catania, Italy.

Abstract

The immunoexpression profile of matrix metalloproteinase-13 was investigated for the first time in dentin of human caries and healthy teeth. Twelve permanent premolars (10 caries and 2 sound) were decalcified in ethylenediaminetetraacetic acid and processed for embedding in paraffin wax. Sections 3-4 µm in thickness were cut and processed for immunohistochemistry. A mouse monoclonal anti-metalloproteinase-13 antibody was used for localisation using an immunoperoxidase technique. Dentinal immunoreactivity was detected in all teeth; it was weak in sound teeth and strong close to the caries area. These in vivo findings suggest a role for metalloproteinase-13 in the development and progression of adult human dental tissue disorders.

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Author Biographies

C. Loreto, University of Catania
Department of Bio-Medical Sciences, Section of Anatomy
C. Galanti, University of Catania
Department of Medical and Surgical Sciences, II Dental Unit
G. Musumeci, University of Catania
Department of Bio-Medical Sciences, Section of Anatomy
M. C. Rusu, "Carol Davila" University of Medicine and Pharmacy
Discipline of Anatomy, Faculty of Dental Medicine
R. Leonardi, University of Catania
Department of Medical and Surgical Sciences, II Dental Unit
Published
2014-03-17
Section
Brief Reports
Keywords:
Human teeth, caries, MMP-13, dentin, immunohistochemistry.
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How to Cite
Loreto, C., Galanti, C., Musumeci, G., Rusu, M. C., & Leonardi, R. (2014). Immunohistochemical analysis of matrix metalloproteinase-13 in human caries dentin. European Journal of Histochemistry, 58(1). https://doi.org/10.4081/ejh.2014.2318

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