Skeletal muscle cellularity and glycogen distribution in the hypermuscular Compact mice

  • T. Kocsis University of Szeged, Hungary.
  • J. Baán University of Szeged, Hungary.
  • G. Müller Egis Pharmaceuticals, Hungary.
  • L. Mendler University of Szeged, Hungary.
  • L. Dux University of Szeged, Hungary.
  • A. Keller-Pintér | keller.aniko@med.u-szeged.hu University of Szeged, Hungary.

Abstract

The TGF-beta member myostatin acts as a negative regulator of skeletal muscle mass. The Compact mice were selected for high protein content and hypermuscularity, and carry a naturally occurring 12-bp deletion in the propeptide region of the myostatin precursor. We aimed to investigate the cellular characteristics and the glycogen distribution of the Compact tibialis anterior (TA) muscle by quantitative histochemistry and spectrophotometry. We have found that the deficiency in myostatin resulted in significantly increased weight of the investigated hindlimb muscles compared to wild type. Although the average glycogen content of the individual fibers kept unchanged, the total amount of glycogen in the Compact TA muscle increased two-fold, which can be explained by the presence of more fibers in Compact compared to wild type muscle. Moreover, the ratio of the most glycolytic IIB fibers significantly increased in the Compact TA muscle, of which glycogen content was the highest among the fast fibers. In summary, myostatin deficiency caused elevated amount of glycogen in the TA muscle but did not increase the glycogen content of the individual fibers despite the marked glycolytic shift observed in Compact mice.

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Author Biographies

T. Kocsis, University of Szeged

Faculty of General Medicine
Department of Biochemistry

J. Baán, University of Szeged

Faculty of General Medicine
Department of Biochemistry

L. Mendler, University of Szeged

Faculty of General Medicine
Department of Biochemistry

L. Dux, University of Szeged

Faculty of General Medicine
Department of Biochemistry

A. Keller-Pintér, University of Szeged

Faculty of General Medicine
Department of Biochemistry

Published
2014-07-28
Section
Original Papers
Supporting Agencies
TÁMOP 4.2.4. A/2-11-1-2012-0001 ‘National Excellence Program’ and TÁMOP-4.2.2.A-11/1/KONV-2012-0035
Keywords:
Compact mice, fiber-type, GDF-8, glycogen, muscle, myostatin.
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How to Cite
Kocsis, T., Baán, J., MüllerG., Mendler, L., Dux, L., & Keller-PintérA. (2014). Skeletal muscle cellularity and glycogen distribution in the hypermuscular Compact mice. European Journal of Histochemistry, 58(3). https://doi.org/10.4081/ejh.2014.2353