Immunocytochemical detection of dentin matrix proteins in primary teeth from patients with dentinogenesis imperfecta associated with osteogenesis imperfecta

  • G. Orsini Polytechnic University of Marche, Italy.
  • A. Majorana University of Brescia, Italy.
  • A. Mazzoni University of Trieste, Italy.
  • A. Putignano Polytechnic University of Marche, Italy.
  • M. Falconi University of Bologna, Italy.
  • A. Polimeni La Sapienza University of Rome, Italy.
  • L. Breschi | lorenzo.breschi@unibo.it University of Bologna, Italy.

Abstract

Dentinogenesis imperfecta determines structural alterations of the collagen structure still not completely elucidated. Immunohistochemical analysis was used to assay Type I and VI collagen, various non-collagenous proteins distribution in human primary teeth from healthy patients or from patients affected by type I dentinogenesis imperfecta (DGI-I) associated with osteogenesis imperfecta (OI). In sound primary teeth, an organized well-known ordered pattern of the type I collagen fibrils was found, whereas atypical and disorganized fibrillar structures were observed in dentin of DGI-I affected patients. Expression of type I collagen was observed in both normal and affected primary teeth, although normal dentin stained more uniformly than DGI-I affected dentin. Reactivity of type VI collagen was significantly lower in normal teeth than in dentin from DGI-I affected patients (P<0.05). Expressions of dentin matrix protein (DMP)-1 and osteopontin (OPN) were observed in both normal dentin and dentin from DGI-I affected patients, without significant differences, being DMP1 generally more abundantly expressed. Immunolabeling for chondroitin sulfate (CS) and biglycan (BGN) was weaker in dentin from DGI-I-affected patients compared to normal dentin, this decrease being significant only for CS. This study shows ultrastructural alterations in dentin obtained from patients affected by DGI-I, supported by immunocytochemical assays of different collagenous and non-collagenous proteins.

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Published
2014-12-01
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Original Papers
Keywords:
Osteogenesis imperfecta, dentinogenesis imperfect, immune electron microscopy, collagen, non-collagenous proteins.
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How to Cite
Orsini, G., Majorana, A., Mazzoni, A., Putignano, A., Falconi, M., Polimeni, A., & Breschi, L. (2014). Immunocytochemical detection of dentin matrix proteins in primary teeth from patients with dentinogenesis imperfecta associated with osteogenesis imperfecta. European Journal of Histochemistry, 58(4). https://doi.org/10.4081/ejh.2014.2405

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