https://doi.org/10.4081/ejh.2014.2408
Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment
Accepted: 24 June 2014
HTML: 564
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The aim of the study was to assess the expression and subcellular localization of visfatin in HCT-116 colorectal carcinoma cells after cytokinesis failure using Cytochalasin B (CytB) and the mechanism of apoptosis of cells after CytB. We observed translocation of visfatin’s antigen in cytB treated colorectal carcinoma HCT-116 cells from cytosol to nucleus. Statistical and morphometric analysis revealed significantly higher area-related numerical density visfatin-bound nano-golds in the nuclei of cytB-treated HCT-116 cells compared to cytosol. Reverse relation to visfatin subcellular localization was observed in un-treated HCT-116 cells. The total amount of visfatin protein and visfatin mRNA level in HCT-116 cells was also decreased after CytB treatment. Additionally, CytB significantly decreased cell survival, increased levels of G2/M fractions, induced bi-nuclei formation as well as increased reactive oxygen species (ROS) level in HCT-116 cells. CytB treatment showed cytotoxic effect that stem from oxidative stress and is connected with the changes in the cytoplasmic/nuclear amount of visfatin in HCT-116 cells.
Supporting Agencies
Silesian Medical University in Katowice, Poland. Grant # KNW-2-009/N/3/N, GCONiI - Upper-Silesian Center for Scientific Computation, grant #POIG.02.03.01-24-099/13.How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- D. Martini, A. Trirè, L. Breschi, A. Mazzoni, G. Teti, M. Falconi, A. Ruggeri Jr, Dentin matrix protein 1 and dentin sialophosphoprotein in human sound and carious teeth: an immunohistochemical and colorimetric assay , European Journal of Histochemistry: Vol. 57 No. 4 (2013)
- S. Salucci, S. Burattini, E. Falcieri, P. Gobbi, Three-dimensional apoptotic nuclear behavior analyzed by means of Field Emission in Lens Scanning Electron Microscope , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- W.J. Liu, J. Yang, Preferentially regulated expression of connexin 43 in the developing spiral ganglion neurons and afferent terminals in post-natal rat cochlea , European Journal of Histochemistry: Vol. 59 No. 1 (2015)
- E.V. Seliverstova, N.P. Prutskova, Receptor-mediated endocytosis of lysozyme in renal proximal tubules of the frog Rana temporaria , European Journal of Histochemistry: Vol. 59 No. 2 (2015)
- H. Zhang, X. Guo, S. Zhong, T. Ge, S. Peng, P. Yu, Z. Zhou, Heterogeneous vesicles in mucous epithelial cells of posterior esophagus of Chinese giant salamander (Andrias davidianus) , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- M. Malatesta, C. Zancanaro, M. Costanzo, B. Cisterna, C. Pellicciari, Simultaneous ultrastructural analysis of fluorochrome-photoconverted diaminobenzidine and gold immunolabeling in cultured cells , European Journal of Histochemistry: Vol. 57 No. 3 (2013)
- Simona Polakovičová, Mária Csӧbӧnyeiová, Barbora Filova, Miroslav Borovský, Ladislav Maršík, Alena Kvasilová, Štefan Polák, Merkel-like cell distribution in the epithelium of the human vagina. An immunohistochemical and TEM study , European Journal of Histochemistry: Vol. 62 No. 1 (2018)
- G. Orsini, A. Majorana, A. Mazzoni, A. Putignano, M. Falconi, A. Polimeni, L. Breschi, Immunocytochemical detection of dentin matrix proteins in primary teeth from patients with dentinogenesis imperfecta associated with osteogenesis imperfecta , European Journal of Histochemistry: Vol. 58 No. 4 (2014)
- S. Marzo, V. Galimberti, M. Biggiogera, Unexpected distribution of KRIT1 inside the nucleus: new insight in a complex molecular pathway , European Journal of Histochemistry: Vol. 58 No. 1 (2014)
- M.L. Escobar, O.M. EcheverrÃa, G. GarcÃa, R. OrtÃz, G.H. Vázquez-Nin, Immunohistochemical and ultrastructural study of the lamellae of oocytes in atretic follicles in relation to different processes of cell death , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
You may also start an advanced similarity search for this article.
