https://doi.org/10.4081/ejh.2014.2408
0
0
0
0
Smart Citations
0
0
0
0
Citing PublicationsSupportingMentioningContrasting
View Citations

See how this article has been cited at scite.ai

scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment

Authors

R.J. Bułdak
rbuldak@sum.edu.pl
Medical University of Silesia, Poland.
M. Skonieczna
Silesian University of Technology, Poland.
Ł. Bułdak
Medical University of Silesia, Poland.
N. Matysiak
Medical University of Silesia, Poland.
Ł. Mielańczyk
Medical University of Silesia, Poland.
G. Wyrobiec
Medical University of Silesia, Poland.
M. Kukla
Medical University of Silesia, Poland.
M. Michalski
Medical University of Silesia, Poland.
K. Żwirska-Korczala
Medical University of Silesia, Poland.

The aim of the study was to assess the expression and subcellular localization of visfatin in HCT-116 colorectal carcinoma cells after cytokinesis failure using Cytochalasin B (CytB) and the mechanism of apoptosis of cells after CytB. We observed translocation of visfatin’s antigen in cytB treated colorectal carcinoma HCT-116 cells from cytosol to nucleus. Statistical and morphometric analysis revealed significantly higher area-related numerical density visfatin-bound nano-golds in the nuclei of cytB-treated HCT-116 cells compared to cytosol. Reverse relation to visfatin subcellular localization was observed in un-treated HCT-116 cells. The total amount of visfatin protein and visfatin mRNA level in HCT-116 cells was also decreased after CytB treatment. Additionally, CytB significantly decreased cell survival, increased levels of G2/M fractions, induced bi-nuclei formation as well as increased reactive oxygen species (ROS) level in HCT-116 cells. CytB treatment showed cytotoxic effect that stem from oxidative stress and is connected with the changes in the cytoplasmic/nuclear amount of visfatin in HCT-116 cells.

Dimensions

Altmetric

PlumX Metrics

Downloads

Publication Facts

Metric
This article
Other articles
Peer reviewers 
2
2.4

Reviewer profiles  N/A

Author statements

Author statements
This article
Other articles
Data availability 
N/A
16%
External funding 
N/A
32%
Competing interests 
N/A
11%
Metric
This journal
Other journals
Articles accepted 
58%
33%
Days to publication 
157
145

Indexed in

Editor & editorial board
profiles
Academic society 
N/A
Publisher 
PAGEPress Publications, Pavia, Italy

PFL

1 2 3 4 5
Not useful Very useful

Citations

Crossref
6
Scopus
0
Google Scholar
Europe PMC
Bartłomiej Płaczek, Rafał J. Bułdak, Andrzej Brenk, Renata Polaniak (2015)
Intelligent Information and Database Systems. Lecture Notes in Computer Science, 9012, 53.
10.1007/978-3-319-15705-4_6
Yangchun Li, Zhaoping Wang, Zongwu Zhao, Yuting Cui (2019)
iTRAQ-based proteome profiling of hyposaline responses in zygotes of the Pacific oyster Crassostrea gigas. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 30, 14.
10.1016/j.cbd.2018.12.003
Xiao-Gang Li, Wei-Dong Pan, Hua-Yong Lou, Ru-Ming Liu, Jian-Hui Xiao, Jian-Jiang Zhong (2015)
New cytochalasins from medicinal macrofungus Crodyceps taii and their inhibitory activities against human cancer cells. Bioorganic & Medicinal Chemistry Letters, 25(9), 1823.
10.1016/j.bmcl.2015.03.059
Hong-Tai Chang, Chiang-Ting Chou, I.-Shu Chen, Chia-Cheng Yu, Ti Lu, Shu-Shong Hsu, Pochuen Shieh, Chung-Ren Jan, Wei-Zhe Liang (2016)
Mechanisms underlying effect of the mycotoxin cytochalasin B on induction of cytotoxicity, modulation of cell cycle, Ca2+ homeostasis and ROS production in human breast cells. Toxicology, 370, 1.
10.1016/j.tox.2016.09.006
Seweryn Gałecki, Patrycja Niesłoń, Daria Kostka, Karol Mierzwa, Magdalena Wȩgrzyn, Daniel Fochtman, Małgorzata Adamiec, Dorota Hudy, Magdalena Skonieczna (2022)
Innovations and Developments of Technologies in Medicine, Biology and Healthcare. Advances in Intelligent Systems and Computing, 1360, 26.
10.1007/978-3-030-88976-0_4
Petr Svoboda, Edita Krizova, Sarka Sestakova, Kamila Vapenkova, Zdenek Knejzlik, Silvie Rimpelova, Diana Rayova, Nikol Volfova, Ivana Krizova, Michaela Rumlova, David Sykora, Rene Kizek, Martin Haluzik, Vaclav Zidek, Jarmila Zidkova, Vojtech Skop (2019)
Nuclear transport of nicotinamide phosphoribosyltransferase is cell cycle–dependent in mammalian cells, and its inhibition slows cell growth. Journal of Biological Chemistry, 294(22), 8676.
10.1074/jbc.RA118.003505

Supporting Agencies

Silesian Medical University in Katowice, Poland. Grant # KNW-2-009/N/3/N, GCONiI - Upper-Silesian Center for Scientific Computation, grant #POIG.02.03.01-24-099/13.
R.J. Bułdak, Medical University of Silesia
Department of Physiology

How to Cite

BuÅ‚dak, R., Skonieczna, M., BuÅ‚dak, Ł., Matysiak, N., MielaÅ„czyk, Ł., Wyrobiec, G., … Å»wirska-Korczala, K. (2014). Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment. European Journal of Histochemistry, 58(3). https://doi.org/10.4081/ejh.2014.2408

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.


Similar Articles

<< < 8 9 10 11 12 13 14 15 16 17 > >> 

You may also start an advanced similarity search for this article.