https://doi.org/10.4081/ejh.2014.2408
Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment
Accepted: 24 June 2014
HTML: 564
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The aim of the study was to assess the expression and subcellular localization of visfatin in HCT-116 colorectal carcinoma cells after cytokinesis failure using Cytochalasin B (CytB) and the mechanism of apoptosis of cells after CytB. We observed translocation of visfatin’s antigen in cytB treated colorectal carcinoma HCT-116 cells from cytosol to nucleus. Statistical and morphometric analysis revealed significantly higher area-related numerical density visfatin-bound nano-golds in the nuclei of cytB-treated HCT-116 cells compared to cytosol. Reverse relation to visfatin subcellular localization was observed in un-treated HCT-116 cells. The total amount of visfatin protein and visfatin mRNA level in HCT-116 cells was also decreased after CytB treatment. Additionally, CytB significantly decreased cell survival, increased levels of G2/M fractions, induced bi-nuclei formation as well as increased reactive oxygen species (ROS) level in HCT-116 cells. CytB treatment showed cytotoxic effect that stem from oxidative stress and is connected with the changes in the cytoplasmic/nuclear amount of visfatin in HCT-116 cells.
Supporting Agencies
Silesian Medical University in Katowice, Poland. Grant # KNW-2-009/N/3/N, GCONiI - Upper-Silesian Center for Scientific Computation, grant #POIG.02.03.01-24-099/13.How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- F Parillo, C Dall’Aglio, A Verini Supplizi, P Ceccarelli, AM Gargiulo, Immunogold study on lectin binding in the porcine zona pellucida and granulosa cells , European Journal of Histochemistry: Vol. 47 No. 4 (2003)
- A Pugnaloni, GL Sgarbi, M Tesei, M D'Aurelio, G Ragni, G Parenti Castelli, S Salardi, S Zucchini, C Bovina, E Cacciari, G Lenaz, G Biagini, Lymphocyte dysmetabolism: an immunocytochemical comparative approach in IDDM and control subjects , European Journal of Histochemistry: Vol. 45 No. 1 (2001)
- K.R. Torres-da-Silva, A.V. da Silva, N.O. Barioni, G.W.L. Tessarin, J.A. de Oliveira, E. Ervolino, J.A.C. Horta-Junior, C.A. Casatti, Neurochemistry study of spinal cord in non-human primate (Sapajus spp.) , European Journal of Histochemistry: Vol. 60 No. 3 (2016)
- Tao Wu, Shikui Wu, Hui Gao, Haolei Liu, Jun Feng, Ge Yin, Astragaloside IV augments anti-PD-1 therapy to suppress tumor growth in lung cancer by remodeling the tumor microenvironment , European Journal of Histochemistry: Vol. 68 No. 4 (2024)
- DL Ribeiro, EJ Caldeira, EM Cândido, AJ Manzato, SR Taboga, VH Cagnon, Prostatic stromal microenvironment and experimental diabetes , European Journal of Histochemistry: Vol. 50 No. 1 (2006)
- S. Battistelli, B. Citterio, B. Baldelli, C. Parlani, M. Malatesta, Histochemical and morphometrical study of mouse intestine epithelium after a long term diet containing genetically modified soybean , European Journal of Histochemistry: Vol. 54 No. 3 (2010)
- S. He, J. Yang, Maturation of neurotransmission in the developing rat cochlea: immunohistochemical evidence from differential expression of synaptophysin and synaptobrevin 2 , European Journal of Histochemistry: Vol. 55 No. 1 (2011)
- L. Casadei, L. Vallorani, A.M. Gioacchini, M. Guescini, S. Burattini, A. D'Emilio, L. Biagiotti, E. Falcieri, V. Stocchi, Proteomics-based investigation in C2C12 myoblast differentiation , European Journal of Histochemistry: Vol. 53 No. 4 (2009)
- Carlo Alberto Redi, Immunoelectron microscopy - Methods and protocols , European Journal of Histochemistry: Vol. 55 No. 3 (2011)
- Y. Sun, L. Zhu, X. Huang, C. Zhou, X. Zhang, Immunohistochemical localization of nerve fibers in the pseudocapsule of fibroids , European Journal of Histochemistry: Vol. 58 No. 2 (2014)
<< < 12 13 14 15 16 17 18 > >>
You may also start an advanced similarity search for this article.
