https://doi.org/10.4081/ejh.2014.2408

Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment

Vol. 58 No. 3 (2014)
Submitted: 7 April 2014
Accepted: 24 June 2014
Published: 12 September 2014
Visfatin, cytochalasin B, immunogold labeling, TEM, adipocytokines.
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Authors

R.J. Bułdak
rbuldak@sum.edu.pl
Medical University of Silesia, Poland.
M. Skonieczna
Silesian University of Technology, Poland.
Ł. Bułdak
Medical University of Silesia, Poland.
N. Matysiak
Medical University of Silesia, Poland.
Ł. Mielańczyk
Medical University of Silesia, Poland.
G. Wyrobiec
Medical University of Silesia, Poland.
M. Kukla
Medical University of Silesia, Poland.
M. Michalski
Medical University of Silesia, Poland.
K. Żwirska-Korczala
Medical University of Silesia, Poland.

The aim of the study was to assess the expression and subcellular localization of visfatin in HCT-116 colorectal carcinoma cells after cytokinesis failure using Cytochalasin B (CytB) and the mechanism of apoptosis of cells after CytB. We observed translocation of visfatin’s antigen in cytB treated colorectal carcinoma HCT-116 cells from cytosol to nucleus. Statistical and morphometric analysis revealed significantly higher area-related numerical density visfatin-bound nano-golds in the nuclei of cytB-treated HCT-116 cells compared to cytosol. Reverse relation to visfatin subcellular localization was observed in un-treated HCT-116 cells. The total amount of visfatin protein and visfatin mRNA level in HCT-116 cells was also decreased after CytB treatment. Additionally, CytB significantly decreased cell survival, increased levels of G2/M fractions, induced bi-nuclei formation as well as increased reactive oxygen species (ROS) level in HCT-116 cells. CytB treatment showed cytotoxic effect that stem from oxidative stress and is connected with the changes in the cytoplasmic/nuclear amount of visfatin in HCT-116 cells.

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Supporting Agencies

Silesian Medical University in Katowice, Poland. Grant # KNW-2-009/N/3/N, GCONiI - Upper-Silesian Center for Scientific Computation, grant #POIG.02.03.01-24-099/13.
R.J. Bułdak, Medical University of Silesia
Department of Physiology

How to Cite

BuÅ‚dak, R., Skonieczna, M., BuÅ‚dak, Ł., Matysiak, N., MielaÅ„czyk, Ł., Wyrobiec, G., … Å»wirska-Korczala, K. (2014). Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment. European Journal of Histochemistry, 58(3). https://doi.org/10.4081/ejh.2014.2408

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