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Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures

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Mechanical stimulation is known to regulate the calcification of endplate chondrocytes. The ANK protein has a strong influence on anti-calcification by transports intracellular inorganic pyrophosphate (PPi) to the extracellular. It is known that TGF-β1 is able to induced Ank gene expression and protect chondrocyte calcification. Intermittent cyclic mechanical tension (ICMT) could induce calcification of endplate chondrocytes by decrease the expression of Ank gene. In this study, we investigated the relation of intermittent cyclic mechanical unconfined compression (ICMC) and Ank gene expression. We found that ICMC decreased the Ank gene expression in the endplate chondrocytes, and there was an decreased in the  TGF-β1 expression after ICMC stimulation. The Ank gene expression significantly increased when treated by transforming growth factor alpha 1 (TGF-β1) in a dose-dependent manner and decreased when treated by SB431542 (ALK inhibitor) in a dose-dependent manner. Our results implicate that ICMC-induced downregulation of Ank gene expression may be regulated by TGF-β1 in endplate chondrocytes.

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Citations

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10.1016/j.heliyon.2024.e31162
Quan Zheng, Chuan-dong Wang, Song Shao, Ming-fan Wu, Qiang-bing Dou, Qi-wei Wang, Liang-ye Sun (2023)
Intermittent cyclic mechanical compression promotes endplate chondrocytes degeneration by disturbing Nrf2/PINK1 signaling pathway-dependent mitophagy. Human Cell, 36(6), 1978.
10.1007/s13577-023-00959-7
Houcheng Zhu, JianHang Ren, Xiangjin Wang, Wenjing Qin, Yong Xie (2025)
Targeting skeletal interoception: a novel mechanistic insight into intervertebral disc degeneration and pain management. Journal of Orthopaedic Surgery and Research, 20(1),
10.1186/s13018-025-05577-7
Liang Xiao, Baiyang Ding, Shujuan Xu, Jianming Gao, Bijing Yang, Jing Wang, Hongguang Xu (2020)
circRNA_0058097 promotes tension‐induced degeneration of endplate chondrocytes by regulating HDAC4 expression through sponge adsorption of miR‐365a‐5p. Journal of Cellular Biochemistry, 121(1), 418.
10.1002/jcb.29202
Li Z. (2022)
Three-dimensional culture of astrocytes in agarose gel matrix. Chinese Journal of Medical Physics, 39(4), 504-509.
10.3969/j.issn.1005-202X.2022.04.020
Sun S. (2021)
The Role and Mechanisms of Mechanical Stimulation in Degeneration of Vertebral Cartilage Endplates. Yiyong Shengwu Lixue/Journal of Medical Biomechanics, 36(4), 652-658.
10.16156/j.1004-7220.2021.04.023
Hashimoto K. (2017)
Lectin-like, oxidized lowdensity lipoprotein receptor-1- deficient mice show resistance to age-related knee osteoarthritis. European Journal of Histochemistry, 61(1), 49-56.
10.4081/ejh.2017.2762
Pellicciari C. (2016)
Is there still room for novelty, in histochemical papers?. European Journal of Histochemistry, 60(4), 285-290.
10.4081/ejh.2016.2758
Mitton-Fitzgerald E. (2016)
The Role of ANK in Calcium Pyrophosphate Deposition Disease. Current Rheumatology Reports, 18(5),
10.1007/s11926-016-0574-z
Pellicciari C. (2015)
Histochemistry in biology and medicine: A message from the citing journals. European Journal of Histochemistry, 59(4), 319-325.
10.4081/ejh.2015.2610
Pellicciari C. (2014)
Impact of Histochemistry on biomedical research: Looking through the articles published in a long-established histochemical journal. European Journal of Histochemistry, 58(4), 338-344.
10.4081/ejh.2014.2474

Supporting Agencies

Chinese National Natural Sciences Fund Project, Chinese Anhui Province Natural Science Fund Project
H.G. Xu, Wannan Medical College
Department of Spine Surgery, Yijishan Hospital
W. Zhang, Wannan Medical College
Department of Orthopedic Surgery, Yijishan Hospital
Q. Zheng, Wannan Medical College
Department of Orthopedic Surgery, Yijishan Hospital
Y.F. Yu, Institute of Health Sciences
The Key Laboratory of Stem Cell Biology
L.F. Deng, Institute of Health Sciences
The Key Laboratory of Stem Cell Biology
H. Wang, Wannan Medical College
Department of Orthopedic Surgery, Yijishan Hospital
P. Liu, Wannan Medical College
Department of Orthopedic Surgery, Yijishan Hospital
M. Zhang, Wannan Medical College
Department of Orthopedic Surgery, Yijishan Hospital

How to Cite

Xu, H., Zhang, W., Zheng, Q., Yu, Y., Deng, L., Wang, H., … Zhang, M. (2014). Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures. European Journal of Histochemistry, 58(3). https://doi.org/10.4081/ejh.2014.2415

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