Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis

  • A.C. Croce | croce@igm.cnr.it Institute of Molecular Genetics of the National Research Council, University of Pavia, Italy.
  • G. Bottiroli Institute of Molecular Genetics of the National Research Council, University of Pavia, Italy.

Abstract

Native fluorescence, or autofluorescence (AF), consists in the emission of light in the UV-visible, near-IR spectral range when biological substrates are excited with light at suitable wavelength. This is a well-known phenomenon, and the strict relationship of many endogenous fluorophores with morphofunctional properties of the living systems, influencing their AF emission features, offers an extremely powerful resource for directly monitoring the biological substrate condition. Starting from the last century, the technological progresses in microscopy and spectrofluorometry were convoying attention of the scientific community to this phenomenon. In the future, the interest in the autofluorescence will certainly continue. Current instrumentation and analytical procedures will likely be overcome by the unceasing progress in new devices for AF detection and data interpretation, while a progress is expected in the search and characterization of endogenous fluorophores and their roles as intrinsic biomarkers.

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Published
2014-12-12
Section
Reviews - 60th Anniversary EJH
Supporting Agencies
Fondazione Cariplo, grant n. 2011-0439
Keywords:
Endogenous-fluorophores, energetic-metabolism, functionality-monitoring.
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How to Cite
Croce, A., & Bottiroli, G. (2014). Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis. European Journal of Histochemistry, 58(4). https://doi.org/10.4081/ejh.2014.2461