https://doi.org/10.4081/ejh.2015.2462

Muc5ac mucin expression during rat skin development

Vol. 59 No. 1 (2015)
Submitted: 21 November 2014
Accepted: 7 January 2015
Published: 20 February 2015
Muc5ac, skin, rat, development
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Authors

V. Ferretti
National University of La Plata, Argentina.
Á. Segal-Eiras
National University of La Plata, Argentina.
C.G. Barbeito
barbeito@fev.unlp.edu.ar
National University of La Plata, Argentina.
M.V. Croce
National University of La Plata, Argentina.

Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during prenatal and postnatal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.Conclusion:  Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.

 

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Supporting Agencies

National University of La Plata
Á. Segal-Eiras, National University of La Plata
Centre of Basic and Applied Immunological Research, Faculty of Medical Sciences, National University of La Plata. Director.
C.G. Barbeito, National University of La Plata
Cathedra of Histology and Embryology, Faculty of Veterinary, UNLP. Full Professor
M.V. Croce, National University of La Plata
Centre for Basic and Applied Immunological Research, Faculty of Medical Sciences, National University of La Plata. Full Professor. Senior Researcher

How to Cite

Ferretti, V., Segal-Eiras, Á., Barbeito, C., & Croce, M. (2015). Muc5ac mucin expression during rat skin development. European Journal of Histochemistry, 59(1). https://doi.org/10.4081/ejh.2015.2462

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