Infection with CagA-positive Helicobacter pylori strain containing three EPIYA C phosphorylation sites is associated with more severe gastric lesions in experimentally infected Mongolian gerbils (Meriones unguiculatus)

  • M. Ferreira Júnior Federal University of Minas Gerais, Brazil.
  • S.A. Batista Federal University of Minas Gerais, Brazil.
  • P.V.T. Vidigal Federal University of Minas Gerais, Brazil.
  • A.A.C. Cordeiro Federal University of Minas Gerais, Brazil.
  • F.M.S. Oliveira Federal University of Minas Gerais, Brazil.
  • L.O. Prata Federal University of Minas Gerais, Brazil.
  • A.E.T. Diniz Federal University of Minas Gerais, Brazil.
  • C.M. Barral Federal University of Minas Gerais, Brazil.
  • R.C. Barbuto Federal University of Minas Gerais, .
  • A.D. Gomes Federal University of Minas Gerais, Brazil.
  • I.D. Araújo Federal University of Minas Gerais, Brazil.
  • D.M.M. Queiroz Federal University of Minas Gerais, Brazil.
  • M.V. Caliari | caliari@icb.ufmg.br Federal University of Minas Gerais, Brazil. http://orcid.org/0000-0001-8354-8407

Abstract

Infection with Helicobacter pylori strains containing high number of EPIYA-C phosphorylation sites in the CagA is associated with significant gastritis and increased risk of developing pre-malignant gastric lesions and gastric carcinoma. However, these findings have not been reproduced in animal models yet. Therefore, we investigated the effect on the gastric mucosa of Mongolian gerbil (Meriones unguiculatus) infected with CagA-positive H. pylori strains exhibiting one or three EPIYA-C phosphorilation sites. Mongolian gerbils were inoculated with H. pylori clonal isolates containing one or three EPIYA-C phosphorylation sites. Control group was composed by uninfected animals challenged with Brucella broth alone. Gastric fragments were evaluated by the modified Sydney System and digital morphometry. Clonal relatedness between the isolates was considered by the identical RAPD-PCR profiles and sequencing of five housekeeping genes, vacA i/d region and of oipA. The other virulence markers were present in both isolates (vacA s1i1d1m1, iceA2, and intact dupA). CagA of both isolates was translocated and phosphorylated in AGS cells. After 45 days of infection, there was a significant increase in the number of inflammatory cells and in the area of the lamina propria in the infected animals, notably in those infected by the CagA-positive strain with three EPIYA-C phosphorylation sites. After six months of infection, a high number of EPIYA-C phosphorylation sites was associated with progressive increase in the intensity of gastritis and in the area of the lamina propria. Atrophy, intestinal metaplasia, and dysplasia were also observed more frequently in animals infected with the CagA-positive isolate with three EPIYA-C sites.  We conclude that infection with H. pylori strain carrying a high number of CagA EPIYA-C phosphorylation sites is associated with more severe gastric lesions in an animal model of H. pylori infection.

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Author Biographies

M. Ferreira Júnior, Federal University of Minas Gerais
Department of General Pathology, Institute of Biological Sciences.
S.A. Batista, Federal University of Minas Gerais
Laboratory of Research in Bacteriology, Faculty of Medicine.
P.V.T. Vidigal, Federal University of Minas Gerais
Department of Anatomical Pathology, Faculty of Medicine.
A.A.C. Cordeiro, Federal University of Minas Gerais
Department of General Pathology, Institute of Biological Sciences.
F.M.S. Oliveira, Federal University of Minas Gerais
Department of General Pathology, Institute of Biological Sciences.
L.O. Prata, Federal University of Minas Gerais
Department of General Pathology, Institute of Biological Sciences.
A.E.T. Diniz, Federal University of Minas Gerais
Surgery Department, Faculty of Medicine.
C.M. Barral, Federal University of Minas Gerais
Surgery Department, Faculty of Medicine.
R.C. Barbuto, Federal University of Minas Gerais
Surgery Department, Faculty of Medicine.
A.D. Gomes, Federal University of Minas Gerais
Laboratory of Research in Bacteriology, Faculty of Medicine
I.D. Araújo, Federal University of Minas Gerais
Surgery Department, Faculty of Medicine.
D.M.M. Queiroz, Federal University of Minas Gerais
Laboratory of Research in Bacteriology, Faculty of Medicine
M.V. Caliari, Federal University of Minas Gerais
Department of General Pathology, Institute of Biological Sciences.
Published
2015-04-27
Section
Original Papers
Supporting Agencies
PRPq/Federal University of Minas Gerais, FAPEMIG, CNPq.
Keywords:
Gastritis, atrophy, intestinal metaplasia, dysplasia, Mongolian gerbil, cagA EPIYA C motif.
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How to Cite
Ferreira Júnior, M., Batista, S., Vidigal, P., Cordeiro, A., Oliveira, F., Prata, L., Diniz, A., Barral, C., Barbuto, R., Gomes, A., Araújo, I., Queiroz, D., & Caliari, M. (2015). Infection with CagA-positive Helicobacter pylori strain containing three EPIYA C phosphorylation sites is associated with more severe gastric lesions in experimentally infected Mongolian gerbils (Meriones unguiculatus). European Journal of Histochemistry, 59(2). https://doi.org/10.4081/ejh.2015.2489