Low ozone concentrations stimulate cytoskeletal organization, mitochondrial activity and nuclear transcription

  • M. Costanzo University of Verona, Italy.
  • B. Cisterna University of Verona, Italy.
  • A. Vella University of Verona, Italy.
  • T. Cestari University of Verona, Italy.
  • V. Covi San Rocco Clinic, Italy.
  • G. Tabaracci San Rocco Clinic, Italy.
  • M. Malatesta | manuela.malatesta@univr.it University of Verona, Italy.

Abstract

Ozone therapy is a modestly invasive procedure based on the regeneration capabilities of low ozone concentrations and used in medicine as an alternative/adjuvant treatment for different diseases. However, the cellular mechanisms accounting for the positive effects of mild ozonization are still largely unexplored. To this aim, in the present study the effects of low ozone concentrations (1 to 20 µg O3/mL O2) on structural and functional cell features have been investigated in vitro by using morphological, morphometrical, cytochemical and immunocytochemical techniques at bright field, fluorescence and transmission electron microscopy. Cells exposed to pure O2 or air served as controls. The results demonstrated that the effects of ozoneadministration are dependent on gas concentration, and the cytoskeletal organization, mitochondrial activity and nuclear transcription may be differently affected. This suggests that, to ensure effective and permanent metabolic cell activation, ozone treatments should take into account the cytological and cytokinetic features of the different tissues. 

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Author Biographies

M. Costanzo, University of Verona
Department of Neurological and Movement Sciences, Anatomy and Histology Section
B. Cisterna, University of Verona
Department of Neurological and Movement Sciences, Anatomy and Histology Section
A. Vella, University of Verona
Department of Pathology and Diagnostics, Immunology Section
T. Cestari, University of Verona
Department of Pathology and Diagnostics, Immunology Section
M. Malatesta, University of Verona
Department of Neurological and Movement Sciences, Anatomy and Histology Section
Published
2015-04-21
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Original Papers
Keywords:
Ozone, in vitro culture, epithelial cells, cell nucleus, cytoskeleton, mitochondria, heat shock proteins
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How to Cite
Costanzo, M., Cisterna, B., Vella, A., Cestari, T., Covi, V., Tabaracci, G., & Malatesta, M. (2015). Low ozone concentrations stimulate cytoskeletal organization, mitochondrial activity and nuclear transcription. European Journal of Histochemistry, 59(2). https://doi.org/10.4081/ejh.2015.2515