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Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde

Vol. 59 No. 3 (2015)
Published: August 5 2015
Fixation, Zinc-ethanol-formalin, Immunohistochemistry, Human brain.
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Authors

D.E. Korzhevskii
https://orcid.org/0000-0002-2456-8165
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine, Russian Federation.
E.G. Sukhorukova
http://orcid.org/0000-0001-5521-7248
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine, Russian Federation.
O.V. Kirik
http://orcid.org/0000-0001-6113-3948
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine, Russian Federation.
I.P. Grigorev
ipg-iem@yandex.ru
http://orcid.org/0000-0002-3535-7638
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine, Russian Federation.
Tissue fixation is critical for immunohistochemistry. Recently, we developed a zinc-ethanol-formalin fixative (ZEF), and the present study was aimed to assess the applicability of the ZEF for the human brain histology and immunohistochemistry and to evaluate the detectability of different antigens in the human brain fixed with ZEF. In total, 11 antigens were tested, including NeuN, neuron-specific enolase, GFAP, Iba-1, calbindin, calretinin, choline acetyltransferase, glutamic acid decarboxylase (GAD65), tyrosine hydroxylase, synaptophysin, and α-tubulin. The obtained data show that: i) the ZEF has potential for use in general histological practice, where detailed characterization of human brain morphology is needed; ii) the antigens tested are well-preserved in the human brain specimens fixed in the ZEF.

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Supporting Agencies

Russian Science Foundation
D.E. Korzhevskii, Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine
Department of General and Specific Morphology, Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Chef

How to Cite



Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde. (2015). European Journal of Histochemistry, 59(3). https://doi.org/10.4081/ejh.2015.2530

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