https://doi.org/10.4081/ejh.2015.2530

Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde

Vol. 59 No. 3 (2015)
Submitted: 23 April 2015
Accepted: 23 July 2015
Published: 5 August 2015
Fixation, Zinc-ethanol-formalin, Immunohistochemistry, Human brain.
Abstract Views: 1787
PDF: 752
HTML: 1379
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

D.E. Korzhevskii
https://orcid.org/0000-0002-2456-8165
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine, Russian Federation.
E.G. Sukhorukova
http://orcid.org/0000-0001-5521-7248
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine, Russian Federation.
O.V. Kirik
http://orcid.org/0000-0001-6113-3948
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine, Russian Federation.
I.P. Grigorev
ipg-iem@yandex.ru
http://orcid.org/0000-0002-3535-7638
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine, Russian Federation.
Tissue fixation is critical for immunohistochemistry. Recently, we developed a zinc-ethanol-formalin fixative (ZEF), and the present study was aimed to assess the applicability of the ZEF for the human brain histology and immunohistochemistry and to evaluate the detectability of different antigens in the human brain fixed with ZEF. In total, 11 antigens were tested, including NeuN, neuron-specific enolase, GFAP, Iba-1, calbindin, calretinin, choline acetyltransferase, glutamic acid decarboxylase (GAD65), tyrosine hydroxylase, synaptophysin, and α-tubulin. The obtained data show that: i) the ZEF has potential for use in general histological practice, where detailed characterization of human brain morphology is needed; ii) the antigens tested are well-preserved in the human brain specimens fixed in the ZEF.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC

Supporting Agencies

Russian Science Foundation
D.E. Korzhevskii, Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine
Department of General and Specific Morphology, Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Chef

How to Cite

Korzhevskii, D., Sukhorukova, E., Kirik, O., & Grigorev, I. (2015). Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde. European Journal of Histochemistry, 59(3). https://doi.org/10.4081/ejh.2015.2530

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.


Similar Articles

<< < 36 37 38 39 40 41 42 43 44 45 > >> 

You may also start an advanced similarity search for this article.