GPX4 and GPX7 over-expression in human hepatocellular carcinoma tissues

  • E. Guerriero Istituto Nazionale Tumori “Fondazione Giovanni Pascale”, IRCCS, Italy.
  • F. Capone Istituto Nazionale Tumori “Fondazione Giovanni Pascale”, IRCCS, Italy.
  • M. Accardo Seconda Università degli Studi di Napoli, Italy.
  • A. Sorice Istituto Nazionale Tumori “Fondazione Giovanni Pascale”, IRCCS, Italy.
  • M. Costantini Stazione Zoologica Anton Dohrn, Italy.
  • G. Colonna Seconda Università degli Studi di Napoli, Italy.
  • G. Castello Istituto Nazionale Tumori “Fondazione Giovanni Pascale”, IRCCS, Italy.
  • S. Costantini | susan.costantini@unina2.it Istituto Nazionale Tumori “Fondazione Giovanni Pascale”, IRCCS, Italy.

Abstract

Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is still one of the most fatal cancers. Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis. The selenium is an essential trace mineral implicated as a key factor in the early stage of cancer and exerts its biological function through the selenoproteins. In the last years our group has been studying the involvement of some selenoproteins in HCC. However, no many data are reported in literature about the correlation between HCC and the glutathione peroxidases (GPXs), both selenium and non selenium-containing GPXs. In this paper we have evaluated the GPX4 and GPX7 expression in some paraffin-embedded tissues from liver biopsy of patients with hepatitis C virus (HCV)-related cirrhosis and HCC by immunohistochemistry and RT-qPCR analysis. Our results evidenced that i) GPX4 and GPX7 had a statistically significant over-expression in HCC tissues compared to cirrhotic counterparts used as non tumor tissues, and ii) their expression was higher in grade III HCC tissues with respect to grade I-II samples. Therefore, we propose to use GPX4 and GPX7 as possible markers for improving HCC diagnosis/prognosis.

Downloads

Download data is not yet available.
Published
2015-12-01
Section
Brief Reports
Keywords:
Hepatocellular carcinoma, GPX4, GPX7, immunohistochemistry, RT-qPCR.
Statistics
Abstract views: 1313

PDF: 349
Supplementary: 140
HTML: 638
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Guerriero, E., Capone, F., Accardo, M., Sorice, A., Costantini, M., Colonna, G., Castello, G., & Costantini, S. (2015). GPX4 and GPX7 over-expression in human hepatocellular carcinoma tissues. European Journal of Histochemistry, 59(4). https://doi.org/10.4081/ejh.2015.2540