Immunohistochemical localization of periostin in human gingiva

  • T. Cobo Instituto Asturiano de Odontología, Spain.
  • A. Obaya University of Oviedo, Spain.
  • S. Cal University of Oviedo, Spain.
  • L. Solares University of Oviedo, Spain.
  • R. Cabo University of Oviedo, Spain.
  • J.A. Vega | javega@uniovi.es University of Oviedo, Spain.
  • J. Cobo Instituto Asturiano de Odontología, Spain.

Abstract

The periostin is a matricellular protein expressed in collagen-rich tissues including some dental and periodontal tissues where it is regulated by mechanical forces, growth factors and cytokines. Interestingly the expression of this protein has been found modified in different gingival pathologies although the expression of periostin in normal human gingiva was never investigated. Here we used Western blot and double immunofluorescence coupled to laser-confocal microscopy to investigated the occurrence and distribution of periostin in different segments of the human gingival in healthy subjects. By Western blot a protein band with an estimated molecular mass of 94 kDa was observed. Periostin was localized at the epithelial-connective tissue junction, or among the fibers of the periodontal ligament, and never co-localized with cytokeratin or vimentin thus suggesting it is an extracellular protein. These results demonstrate the occurrence of periostin in adult human gingiva; its localization suggests a role in the bidirectional interactions between the connective tissue and the epithelial cells, and therefore in the physiopathological conditions in which these interactions are altered.  

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Author Biographies

A. Obaya, University of Oviedo
Departamento de Biologia Funcional - Fisiologia
S. Cal, University of Oviedo
Departamento de Bioquimica y Biologia Molecular
L. Solares, University of Oviedo
Departamento de Biolquimica y Biología Molecular
R. Cabo, University of Oviedo
Departamento de Morfología y Biologia Celular
J.A. Vega, University of Oviedo
Dep.of Morphology and Cell Bilogy
Published
2015-09-29
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Original Papers
Supporting Agencies
Instituto Asturiano de Odontología
Keywords:
Periostin, matricellular proteins, human gingiva.
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How to Cite
Cobo, T., Obaya, A., Cal, S., Solares, L., Cabo, R., Vega, J., & Cobo, J. (2015). Immunohistochemical localization of periostin in human gingiva. European Journal of Histochemistry, 59(3). https://doi.org/10.4081/ejh.2015.2548