Changes in muscularis propria of anterior vaginal wall in women with pelvic organ prolapse

  • A. Vetuschi University of L'Aquila, Italy.
  • A. D'Alfonso University of L'Aquila, Italy.
  • R. Sferra | roberta.sferra@univaq.it University of L'Aquila, Italy.
  • D. Zanelli University of L'Aquila, Italy.
  • S. Pompili University of L'Aquila, Italy.
  • F. Patacchiola University of L'Aquila, Italy.
  • E. Gaudio Sapienza University of Roma, Italy.
  • G. Carta University of L'Aquila, Italy.

Abstract

The objective of this study was to evaluate the morphological and immunohistochemical alterations of tissue removed from the upper third of anterior vaginal wall in a sample group of the female population presenting homogenous risk factors associated with Pelvic Organ Prolapse (POP). The case study consisted of 14 patients with POP and there were 10 patients in the control group. Patient selection was carried on the basis of specific criteria and all of the patients involved in the study presented one or more of the recognized POP risk factors. Samples were taken from POP patients during vaginal plastic surgery following  colpohysterectomy, and from control patients during closure of the posterior fornix following hysterectomy. Samples were processed for histological and  immunohistochemical analyses for Collagen I and Collagen III, α-Smooth Muscle Actin (α-SMA), Platelet-Derived-Growth-Factor (PDGF), matrix metalloproteinase 3 (MMP3), Caspase3. Immunofluorescence analyses for Collagen I and III and PDGF were also carried out. In prolapsed specimens our results show a disorganization of smooth muscle cells that appeared to have been displaced by an increased collagen III deposition resulting in rearrangement of the muscularis propria architecture. These findings suggest that the increase in the expression of collagen fibers in muscularis could probably due to a phenotypic switch resulting in the dedifferentiation of smooth muscle cells into myofibroblasts. These alterations could be responsible for the compromising of the dynamic functionality of the pelvic floor.

 

Downloads

Download data is not yet available.
Published
2016-02-16
Section
Original Papers
Keywords:
Pelvic organ prolapse, immunohistochemistry, smooth muscle cells, collagen, PDGF.
Statistics
Abstract views: 841

PDF: 286
HTML: 605
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Vetuschi, A., D’Alfonso, A., Sferra, R., Zanelli, D., Pompili, S., Patacchiola, F., Gaudio, E., & Carta, G. (2016). Changes in muscularis propria of anterior vaginal wall in women with pelvic organ prolapse. European Journal of Histochemistry, 60(1). https://doi.org/10.4081/ejh.2016.2604

Most read articles by the same author(s)