The knee joint loose body as a source of viable autologous human chondrocytes

Abstract

Loose bodies are fragments of cartilage or bone present in the synovial fluid. In the present study we assessed if loose bodies could be used as a source of autologous human chondrocytes for experimental purposes. Histochemical examination of loose bodies and differential enzymatic digestions were undertaken, the isolated cells were cultured in alginate bead microspheres and immunolocalisations were undertaken for chondrogenic markers such as aggrecan, and type II collagen. Isolated loose body cells had high viability (≥90% viable), expressed chondrogenic markers (aggrecan, type II collagen) but no type I collagen. Loose bodies may be a useful source of autologous chondrocytes of high viability.

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Published
2016-06-10
Section
Brief Reports
Supporting Agencies
NHMRC Project Grant 352562
Keywords:
Loose body, human chondrocytes, total knee arthroplasty, repair medicine.
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How to Cite
Melrose, J. (2016). The knee joint loose body as a source of viable autologous human chondrocytes. European Journal of Histochemistry, 60(2). https://doi.org/10.4081/ejh.2016.2645