https://doi.org/10.4081/ejh.2016.2645
The knee joint loose body as a source of viable autologous human chondrocytes
Accepted: 5 May 2016
HTML: 374
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Loose bodies are fragments of cartilage or bone present in the synovial fluid. In the present study we assessed if loose bodies could be used as a source of autologous human chondrocytes for experimental purposes. Histochemical examination of loose bodies and differential enzymatic digestions were undertaken, the isolated cells were cultured in alginate bead microspheres and immunolocalisations were undertaken for chondrogenic markers such as aggrecan, and type II collagen. Isolated loose body cells had high viability (≥90% viable), expressed chondrogenic markers (aggrecan, type II collagen) but no type I collagen. Loose bodies may be a useful source of autologous chondrocytes of high viability.
Supporting Agencies
NHMRC Project Grant 352562How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- M. Ferreira Júnior, S.A. Batista, P.V.T. Vidigal, A.A.C. Cordeiro, F.M.S. Oliveira, L.O. Prata, A.E.T. Diniz, C.M. Barral, R.C. Barbuto, A.D. Gomes, I.D. Araújo, D.M.M. Queiroz, M.V. Caliari, Infection with CagA-positive Helicobacter pylori strain containing three EPIYA C phosphorylation sites is associated with more severe gastric lesions in experimentally infected Mongolian gerbils (Meriones unguiculatus) , European Journal of Histochemistry: Vol. 59 No. 2 (2015)
- Cristina Pirrone, Anna M. Chiaravalli, Alessandro Marando, Andrea Conti, Alessia Rainero, Andrea Pistochini, Francesco Lo Curto, Francesco Pasquali, Paolo Castelnuovo, Carlo Capella, Giovanni Porta, OTX1 and OTX2 as possible molecular markers of sinonasal carcinomas and olfactory neuroblastomas , European Journal of Histochemistry: Vol. 61 No. 1 (2017)
- L. Vinci, A. Ravarino, V. Fanos, A.G. Naccarato, G. Senes, C. Gerosa, G. Bevilacqua, G. Faa, R. Ambu, Immunohistochemical markers of neural progenitor cells in the early embryonic human cerebral cortex , European Journal of Histochemistry: Vol. 60 No. 1 (2016)
- W. Meyer, M. Liumsiricharoen, A. Suprasert, L.G. Fleischer, M. Hewicker-Trautwein, Immunohistochemical demonstration of keratins in the epidermal layers of the Malayan pangolin (Manis javanica), with remarks on the evolution of the integumental scale armour , European Journal of Histochemistry: Vol. 57 No. 3 (2013)
- C. Loreto, C. Galanti, G. Musumeci, M. C. Rusu, R. Leonardi, Immunohistochemical analysis of matrix metalloproteinase-13 in human caries dentin , European Journal of Histochemistry: Vol. 58 No. 1 (2014)
- Gianpaolo Antonio Basile, Salvatore Bertino, Alessia Bramanti, Rosella Ciurleo, Giuseppe Pio Anastasi, Demetrio Milardi, Alberto Cacciola, Striatal topographical organization: Bridging the gap between molecules, connectivity and behavior , European Journal of Histochemistry: Vol. 65 No. s1 (2021): Special Collection on Advances in Neuromorphology in Health and Disease
- Manuel Scimeca, Simone Bischetti, Harpreet Kaur Lamsira, Rita Bonfiglio, Elena Bonanno, Energy Dispersive X-ray (EDX) microanalysis: A powerful tool in biomedical research and diagnosis , European Journal of Histochemistry: Vol. 62 No. 1 (2018)
- R. Leonardi, R.E. Perrotta, C. Loreto, G. Musumeci, S. Crimi, J.N. dos Santos, M.C. Rusu, P. Bufo, E. Barbato, G. Pannone, Toll-like receptor 4 expression in the epithelium of inflammatory periapical lesions. An immunohistochemical study , European Journal of Histochemistry: Vol. 59 No. 4 (2015)
- J. Hansson, A.E. Ericsson, H. Axelson, M.E. Johansson, Species diversity regarding the presence of proximal tubular progenitor cells of the kidney , European Journal of Histochemistry: Vol. 60 No. 1 (2016)
- J. Suchánková, S. Legartová, P. Sehnalová, S. Kozubek, S. Valente, D. Labella, A. Mai, C. Eckerich, F.O. Fackelmayer, D.V. Sorokin, E. Bartova, PRMT1 arginine methyltransferase accumulates in cytoplasmic bodies that respond to selective inhibition and DNA damage , European Journal of Histochemistry: Vol. 58 No. 2 (2014)
<< < 1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.
