NO-tryptophan: a new small molecule located in the rat brain

  • A. Mangas | amangas@gemacbio.com Gemacbio -Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT) - University of Salamanca, Spain.
  • J. Yajeya University of Salamanaca, Department of Physiology, Spain.
  • N. González Gemacbio, France.
  • S. Duleu Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT), France.
  • M. Geffard Gemacbio -Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT), France.
  • R. Coveñas University of Salamanca, Institute of Neurosciences of Castilla y León (INCYL), Laboratory 14, Spain.

Abstract

A highly specific monoclonal antibody directed against nitric oxide-tryptophan (NO-W) with good affinity (10-9 M) and specificity was developed. In the rat brain, using an indirect immunoperoxidase technique, cell bodies containing NO-W were exclusively found in the intermediate and dorsal parts of the lateral septal nucleus. No immunoreactive fibres were found in the rat brain. This work reports the first visualization and the morphological characteristics of cell bodies containing NO-W in the mammalian brain. The restricted distribution of NO-W in the rat brain suggests that this molecule could be involved in specific physiological mechanisms. 

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Author Biographies

A. Mangas, Gemacbio -Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT) - University of Salamanca

Institute of Neurosciences of Castilla y León (INCYL), Laboratory 14

J. Yajeya, University of Salamanaca, Department of Physiology

School of Medicine, Department of Physiology

N. González, Gemacbio

Research Department

S. Duleu, Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT)

Research Department

M. Geffard, Gemacbio -Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT)

Research Department

R. Coveñas, University of Salamanca, Institute of Neurosciences of Castilla y León (INCYL), Laboratory 14

Institute of Neurosciences of Castilla y León (INCYL), Laboratory 14

Published
2016-09-22
Info
Issue
Section
Original Papers
Supporting Agencies
European Union FP7 Collaborative Grant TargetBraIn, GEMAC S.A. Laboratories, IDRPHT
Keywords:
Lateral septal nucleus, septum, monoclonal antibody, cell body, nitric oxide, immunohistochemistry.
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How to Cite
Mangas, A., Yajeya, J., González, N., Duleu, S., Geffard, M., & Coveñas, R. (2016). NO-tryptophan: a new small molecule located in the rat brain. European Journal of Histochemistry, 60(3). https://doi.org/10.4081/ejh.2016.2692