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NO-tryptophan: a new small molecule located in the rat brain

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A highly specific monoclonal antibody directed against nitric oxide-tryptophan (NO-W) with good affinity (10-9 M) and specificity was developed. In the rat brain, using an indirect immunoperoxidase technique, cell bodies containing NO-W were exclusively found in the intermediate and dorsal parts of the lateral septal nucleus. No immunoreactive fibres were found in the rat brain. This work reports the first visualization and the morphological characteristics of cell bodies containing NO-W in the mammalian brain. The restricted distribution of NO-W in the rat brain suggests that this molecule could be involved in specific physiological mechanisms. 

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Supporting Agencies

European Union FP7 Collaborative Grant TargetBraIn, GEMAC S.A. Laboratories, IDRPHT
A. Mangas, Gemacbio -Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT) - University of Salamanca

Institute of Neurosciences of Castilla y León (INCYL), Laboratory 14

J. Yajeya, University of Salamanaca, Department of Physiology

School of Medicine, Department of Physiology

N. González, Gemacbio

Research Department

S. Duleu, Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT)

Research Department

M. Geffard, Gemacbio -Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT)

Research Department

R. Coveñas, University of Salamanca, Institute of Neurosciences of Castilla y León (INCYL), Laboratory 14

Institute of Neurosciences of Castilla y León (INCYL), Laboratory 14

How to Cite



NO-tryptophan: a new small molecule located in the rat brain. (2016). European Journal of Histochemistry, 60(3). https://doi.org/10.4081/ejh.2016.2692