3-hydroxi-anthranilic acid is early expressed in stroke

  • A. Mangas | amangas@gemacbio.com Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT) - University of Salamanca, France.
  • J. Yajeya University of Salamanca, Department of Physiology, Spain.
  • N. González Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT), France.
  • I. Ruiz Gemacbio, France.
  • M. Geffard Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT), France.
  • R. Coveñas University of Salamanca, Institute of Neurosciences of Castilla y León (INCYL), Laboratory of Neuroanatomy of the Peptidergic Systems, Spain.

Abstract

Using an immunohistochemical technique, we have studied the distribution of 3-OH-anthranilic acid (3-HAA) in the rat brain. Our study was carried out in control animals and in rats in which a stroke model (single transient middle cerebral artery occlusion) was performed. A monoclonal antibody directed against 3-HAA was also developed. 3-HAA was exclusively observed in the infarcted regions (ipsilateral striatum/cerebral cortex), 2, 5 and 21 days after the induction of stroke. In control rats and in the contralateral side of the stroke animals, no immunoreactivity for 3-HAA was visualized. Under pathological conditions (from early phases of stroke), we reported for the first time the presence of 3-HAA in the mammalian brain. By double immunohistochemistry, the coexistence of 3-HAA and GFAP was observed in astrocytes. The distribution of 3-HAA matched perfectly with the infarcted regions. Our findings suggest that, in stroke, 3-HAA could be involved in the tissue damage observed in the infarcted regions, since it is well known that 3-HAA exerts cytotoxic effects.

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Author Biographies

A. Mangas, Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT) - University of Salamanca
Research Department Chief
Antibodies Department Manager
J. Yajeya, University of Salamanca, Department of Physiology

School of Medicine, Department of Physiology

Professor

N. González, Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT)

Research Dept

Technician

I. Ruiz, Gemacbio

Research Dept

Technician

 

M. Geffard, Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT)

Chief Scientific Officer

Published
2016-12-02
Info
Issue
Section
Original Papers
Supporting Agencies
GEMAC S.A. Laboratories (Saint Jean d’Illac, France), IDRPHT (Talence, European Union FP7 Collaborative Grant TargetBraIn (number 279017)
Keywords:
Astrocyte, ischemia, glia, immunohistochemistry, monoclonal antibody, neurotoxicity, 3-OH-anthranilic acid.
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How to Cite
Mangas, A., Yajeya, J., González, N., Ruiz, I., Geffard, M., & Coveñas, R. (2016). 3-hydroxi-anthranilic acid is early expressed in stroke. European Journal of Histochemistry, 60(4). https://doi.org/10.4081/ejh.2016.2709