Hormone receptor expression in human fascial tissue

  • C. Fede University of Padua, Department of Neuroscience, Italy.
  • G. Albertin University of Padua, Department of Neuroscience, Italy.
  • L. Petrelli University of Padua, Department of Neuroscience, Italy.
  • M.M. Sfriso University of Padua, Department of Neuroscience, Italy.
  • C. Biz University of Padua, Department of Surgery, Oncology and Gastroenterology DiSCOG, Orthopedic Clinic, Italy.
  • R. De Caro University of Padua, Department of Neuroscience, Italy.
  • C. Stecco | carla.stecco@unipd.it University of Padua, Department of Neuroscience, Italy.

Abstract

Many epidemiologic, clinical, and experimental findings point to sex differences in myofascial pain in view of the fact that adult women tend to have more myofascial problems with respect to men. It is possible that one of the stimuli to sensitization of fascial nociceptors could come from hormonal factors such as estrogen and relaxin, that are involved in extracellular matrix and collagen remodeling and thus contribute to functions of myofascial tissue. Immunohistochemical and molecular investigations (real-time PCR analysis) of relaxin receptor 1 (RXFP1) and estrogen receptor-alpha (ERα) localization were carried out on sample of human fascia collected from 8 volunteers patients during orthopedic surgery (all females, between 42 and 70 yrs, divided into pre- and post-menopausal groups), and in fibroblasts isolated from deep fascia, to examine both protein and RNA expression levels. We can assume that the two sex hormone receptors analyzed are expressed in all the human fascial districts examined and in fascial fibroblasts culture cells, to a lesser degree in the post-menopausal with respect to the pre-menopausal women. Hormone receptor expression was concentrated in the fibroblasts, and RXFP1 was also evident in blood vessels and nerves. Our results are the first demonstrating that the fibroblasts located within different districts of the muscular fasciae express sex hormone receptors and can help to explain the link between hormonal factors and myofascial pain. It is known, in fact, that estrogen and relaxin play a key role in extracellular matrix remodeling by inhibiting fibrosis and inflammatory activities, both important factors affecting fascial stiffness and sensitization of fascial nociceptors.

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Published
2016-11-02
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Original Papers
Keywords:
ia, fascial fibroblasts, estrogen, relaxin, immunostaining.
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How to Cite
Fede, C., Albertin, G., Petrelli, L., Sfriso, M., Biz, C., De Caro, R., & Stecco, C. (2016). Hormone receptor expression in human fascial tissue. European Journal of Histochemistry, 60(4). https://doi.org/10.4081/ejh.2016.2710