Functional topography of the fully grown human oocyte

  • Manuela Monti | m.monti@smatteo.pv.it San Matteo foundation for health, hospitalization and care, Research Center for Regenerative Medicine, Italy.
  • Alberto Calligaro University of Pavia, Department of Public Health, Experimental and Forensic Medicine, Italy.
  • Barry Behr Stanford University, Department of Obstetrics and Gynecology, United States.
  • Renee Rejo Pera Stanford University, Department of Obstetrics and Gynecology, United States.
  • Carlo Alberto Redi University of Pavia, Department of Biology and Biotechnology “L. Spallanzani”, Italy.
  • Mark Wossidlo Stanford University, Institute for Stem Cell Biology and Regenerative Medicine, United States.

Abstract

In vivo maturation (IVM) of human oocytes is a technique used to increase the number of usable oocytes for in vitro fertilization (IVF) and represents a necessity for women with different ovarian pathologies. During IVM the oocytes progress from the germinal vesicle stage (GV) through the metaphase II and during this journey both nuclear and cytoplasmic rearrangements must be obtained to increase the probability to get viable and healthy zygotes/embryos after IVF. As the successful clinical outcomes of this technique are a reality, we wanted to investigate the causes behind oocytes maturation arrest. For obvious ethical reasons, we were able to analyze only few human immature oocytes discarded and donated to research by transmission electron microscopy showing that, as in the mouse, they have different chromatin and cytoplasmic organizations both essential for further embryo development.

Downloads

Download data is not yet available.

Author Biography

Manuela Monti, San Matteo foundation for health, hospitalization and care, Research Center for Regenerative Medicine
Biotechnology Lab
Published
2017-02-06
Section
Original Papers
Keywords:
Human oocytes, human antral compartment, lipid droplets, SN oocytes, NSN oocytes, cytoplasmic lattices, transmission electron microscopy.
Statistics
Abstract views: 949

PDF: 298
HTML: 234
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Monti, M., Calligaro, A., Behr, B., Rejo Pera, R., Redi, C. A., & Wossidlo, M. (2017). Functional topography of the fully grown human oocyte. European Journal of Histochemistry, 61(1). https://doi.org/10.4081/ejh.2017.2769