https://doi.org/10.4081/ejh.2017.2769

Functional topography of the fully grown human oocyte

Vol. 61 No. 1 (2017)
Submitted: 22 December 2016
Accepted: 31 January 2017
Published: 6 February 2017
Human oocytes, human antral compartment, lipid droplets, SN oocytes, NSN oocytes, cytoplasmic lattices, transmission electron microscopy.
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Authors

Manuela Monti
m.monti@smatteo.pv.it
San Matteo foundation for health, hospitalization and care, Research Center for Regenerative Medicine, Italy.
Alberto Calligaro
University of Pavia, Department of Public Health, Experimental and Forensic Medicine, Italy.
Barry Behr
Stanford University, Department of Obstetrics and Gynecology, United States.
Renee Rejo Pera
Stanford University, Department of Obstetrics and Gynecology, United States.
Carlo Alberto Redi
University of Pavia, Department of Biology and Biotechnology “L. Spallanzani”, Italy.
Mark Wossidlo
Stanford University, Institute for Stem Cell Biology and Regenerative Medicine, United States.

In vivo maturation (IVM) of human oocytes is a technique used to increase the number of usable oocytes for in vitro fertilization (IVF) and represents a necessity for women with different ovarian pathologies. During IVM the oocytes progress from the germinal vesicle stage (GV) through the metaphase II and during this journey both nuclear and cytoplasmic rearrangements must be obtained to increase the probability to get viable and healthy zygotes/embryos after IVF. As the successful clinical outcomes of this technique are a reality, we wanted to investigate the causes behind oocytes maturation arrest. For obvious ethical reasons, we were able to analyze only few human immature oocytes discarded and donated to research by transmission electron microscopy showing that, as in the mouse, they have different chromatin and cytoplasmic organizations both essential for further embryo development.

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Manuela Monti, San Matteo foundation for health, hospitalization and care, Research Center for Regenerative Medicine
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How to Cite

Monti, M., Calligaro, A., Behr, B., Rejo Pera, R., Redi, C. A., & Wossidlo, M. (2017). Functional topography of the fully grown human oocyte. European Journal of Histochemistry, 61(1). https://doi.org/10.4081/ejh.2017.2769

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