Expression of the HPV18/E6 oncoprotein induces DNA damage

  • Elva I. Cortés Gutiérrez | elvacortes@cibinmty.net Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica del Noreste, Mexico.
  • Catalina Garcí­a-Vielma Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica del Noreste, Mexico.
  • Adriana Aguilar-Lemarroy Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Occidente, Mexico.
  • Veronica Vallejo-Ruí­z Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Occidente, Mexico.
  • Patricia Piña-Sánchez Instituto Mexicano del Seguro Social, Unidad de Investigación Médica en Enfermedades Oncológicas, Mexico.
  • Pablo Zapata-Benavides Universidad Autónoma de Nuevo León, Faculty of Biology, Mexico.
  • Jaime Gosalvez Universidad Autónoma de Madrid, Department of Biology, Spain.

Abstract

This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.

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Published
2017-06-15
Info
Issue
Section
Original Papers
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).
Keywords:
DNA damage, DNA breakage detection-fluorescence in situ hybridization, DBD-FISH, human papillomavirus, HPV, E6 oncogene, alkaline-labile sites.
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How to Cite
Cortés Gutiérrez, E. I., Garcí­a-VielmaC., Aguilar-Lemarroy, A., Vallejo-Ruí­zV., Piña-Sánchez, P., Zapata-Benavides, P., & Gosalvez, J. (2017). Expression of the HPV18/E6 oncoprotein induces DNA damage. European Journal of Histochemistry, 61(2). https://doi.org/10.4081/ejh.2017.2773