https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 198
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- Yan Yan Li, Ya Ping Feng, Li Liu, Jin Ke, Xing Long, Inhibition of HMGB1 suppresses inflammation and catabolism in temporomandibular joint osteoarthritis via NF-κB signaling pathway , European Journal of Histochemistry: Vol. 66 No. 3 (2022)
- Francesca Diomede, Maria Zingariello, Marcos F.X.B. Cavalcanti, Ilaria Merciaro, Jacopo Pizzicannella, Natalia de Isla, Sergio Caputi, Patrizia Ballerini, Oriana Trubiani, MyD88/ERK/NFkB pathways and pro-inflammatory cytokines release in periodontal ligament stem cells stimulated by Porphyromonas gingivalis , European Journal of Histochemistry: Vol. 61 No. 2 (2017)
- Jana Oswald, Maximilian Büttner, Simon Jasinski-Bergner, Roland Jacobs, Philip Rosenstock, Heike Kielstein, Leptin affects filopodia and cofilin in NK-92 cells in a dose- and time-dependent manner , European Journal of Histochemistry: Vol. 62 No. 1 (2018)
- Zhao Zhang, Hongming Fan, William Richardson, Bruce Z. Gao, Tong Ye, Management of autofluorescence in formaldehyde-fixed myocardium: choosing the right treatment , European Journal of Histochemistry: Vol. 67 No. 4 (2023)
- J. Zhang, J. Luo, J. Ni, L. Tang, H.P. Zhang, L. Zhang, J.F. Xu, D. Zheng, MMP-7 is upregulated by COX-2 and promotes proliferation and invasion of lung adenocarcinoma cells , European Journal of Histochemistry: Vol. 58 No. 1 (2014)
- Minkai Cao, Deping Yuan, Hongxiu Jiang, Guanlun Zhou, Chao Chen, Guorong Han, Long non-coding RNA WAC antisense RNA 1 mediates hepatitis B virus replication in vitro by reinforcing miR-192-5p/ATG7-induced autophagy , European Journal of Histochemistry: Vol. 66 No. 3 (2022)
- T. Karaca, F. Bayiroglu, M.U. Yoruk, S. Kaya, S. Uslu, B. Comba, L. Mis, Effect of royal jelly on experimental colitis Induced by acetic acid and alteration of mast cell distribution in the colon of rats , European Journal of Histochemistry: Vol. 54 No. 4 (2010)
- E. Lacunza, V. Ferretti, C. Barbeito, A. Segal-Eiras, M. V. Croce, Immunohistochemical evidence of Muc1 expression during rat embryonic development , European Journal of Histochemistry: Vol. 54 No. 4 (2010)
- Valeriia Gusel'nikova, Olga Antimonova, Elena Fedorova, Mikhail Shavlovsky, Aleksandr Krutikov, Ekaterina Mikhailova, Aleksandra Gudkova, Vyacheslav Mikhailov, Dmitry Korzhevskii, Fluorescent characterization of amyloid deposits in the kidneys of mdx mice , European Journal of Histochemistry: Vol. 62 No. 2 (2018)
- Yuan Cao, Dong-Hui Ao, Chao Ma, Wen-Ying Qiu, Yi-Cheng Zhu, Immunoreactivity and a new staining method of monocarboxylate transporter 1 located in endothelial cells of cerebral vessels of human brain in distinguishing cerebral venules from arterioles , European Journal of Histochemistry: Vol. 65 No. s1 (2021): Special Collection on Advances in Neuromorphology in Health and Disease
<< < 12 13 14 15 16 17 18 19 20 21 > >>
You may also start an advanced similarity search for this article.
