https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 198
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- V. Poletto, V. Galimberti, G. Guerra, V. Rosti, F. Moccia, M. Biggiogera, Fine structural detection of calcium ions by photoconversion , European Journal of Histochemistry: Vol. 60 No. 3 (2016)
- Zhongzhu Tang, Lei Wang, Yunwang Chen, Xiaomin Zheng, Runyu Wang, Bingxue Liu, Shiqi Zhang, Huimin Wang, Quercetin reverses 5-fluorouracil resistance in colon cancer cells by modulating the NRF2/HO-1 pathway , European Journal of Histochemistry: Vol. 67 No. 3 (2023)
- Chiara Rita Inguscio, Flavia Carton, Barbara Cisterna, Manuela Rizzi, Francesca Boccafoschi, Gabriele Tabaracci, Manuela Malatesta, Low ozone concentrations do not exert cytoprotective effects on tamoxifen-treated breast cancer cells in vitro , European Journal of Histochemistry: Vol. 68 No. 3 (2024)
- S. Grecchi, M. Malatesta, Visualizing endocytotic pathways at transmission electron microscopy via diaminobenzidine photo-oxidation by a fluorescent cell-membrane dye , European Journal of Histochemistry: Vol. 58 No. 4 (2014)
- G.A. Caprara, S. Perni, C. Morabito, M.A. Mariggiò, S. Guarnieri, Specific association of growth-associated protein 43 with calcium release units in skeletal muscles of lower vertebrates , European Journal of Histochemistry: Vol. 58 No. 4 (2014)
- Guoyue Liu, Cunzhi Yin, Mingjiang Qian, Xuan Xiao, Hang Wu, Fujian Fu, LncRNA gadd7 promotes mitochondrial membrane potential decrease and apoptosis of alveolar type II epithelial cells by positively regulating MFN1 in an in vitro model of hyperoxia-induced acute lung injury , European Journal of Histochemistry: Vol. 67 No. 2 (2023)
- J. Knabl, A. Vattai, R. Hüttenbrenner, S. Hutter, M. Karsten, U. Jeschke, RXRα is upregulated in first trimester endometrial glands of spontaneous abortions unlike LXR and PPARγ , European Journal of Histochemistry: Vol. 60 No. 4 (2016)
- C.A. May, I. Osterland, Merkel cell distribution in the human eyelid , European Journal of Histochemistry: Vol. 57 No. 4 (2013)
- Jindong Li, Jie Kang, Weiyan Liu, Jiazhe Liu, Gaofeng Pan, Anwei Mao, Qing Zhang, Jingfeng Lu, Junbin Ding, Hongchang Li, Docetaxel-resistant triple-negative breast cancer cell-derived exosomal lncRNA LINC00667 reduces the chemosensitivity of breast cancer cells to docetaxel via targeting miR-200b-3p/Bcl-2 axis , European Journal of Histochemistry: Vol. 66 No. 4 (2022)
- M. Giagnacovo, R. Cardani, G. Meola, C. Pellicciari, M. Malatesta, Routinely frozen biopsies of human skeletal muscle are suitable for morphological and immunocytochemical analyses at transmission electron microscopy , European Journal of Histochemistry: Vol. 54 No. 3 (2010)
<< < 13 14 15 16 17 18 19 20 21 22 > >>
You may also start an advanced similarity search for this article.
