https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 198
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- D. Martini, A. Trirè, L. Breschi, A. Mazzoni, G. Teti, M. Falconi, A. Ruggeri Jr, Dentin matrix protein 1 and dentin sialophosphoprotein in human sound and carious teeth: an immunohistochemical and colorimetric assay , European Journal of Histochemistry: Vol. 57 No. 4 (2013)
- M. Úbeda-Manzanaro, J.B. Ortiz-Delgado, C. Sarasquete, The Bromodomain testis-specific gene (Brdt) characterization and expression in gilthead seabream, Sparus aurata, and European seabass, Dicentrarchus labrax , European Journal of Histochemistry: Vol. 60 No. 2 (2016)
- Simona Polakovičová, Mária Csӧbӧnyeiová, Barbora Filova, Miroslav Borovský, Ladislav Maršík, Alena Kvasilová, Štefan Polák, Merkel-like cell distribution in the epithelium of the human vagina. An immunohistochemical and TEM study , European Journal of Histochemistry: Vol. 62 No. 1 (2018)
- K. Fujikawa, T. Yokohama-Tamaki, T. Morita, O. Baba, C. Qin, S. Shibata, An in situ hybridization study of perlecan, DMP1, and MEPE in developing condylar cartilage of the fetal mouse mandible and limb bud cartilage , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- M. Derenzini, A. L. Olins, D. E. Olins, Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry , European Journal of Histochemistry: Vol. 58 No. 1 (2014)
- A. Porzionato, M. M. Sfriso, V. Macchi, A. Rambaldo, G. Lago, L. Lancerotto, V. Vindigni, R. De Caro, Decellularized omentum as novel biologic scaffold for reconstructive surgery and regenerative medicine , European Journal of Histochemistry: Vol. 57 No. 1 (2013)
- J. Gosalvez, F. Crespo, J.L. Vega-Pla, C. López-Fernández, E.I. Cortés-Gutiérrez, R. Mezzanotte, Shared Y chromosome repetitive DNA sequences in stallion and donkey as visualized using whole-genomic comparative hybridization , European Journal of Histochemistry: Vol. 54 No. 1 (2010)
- Hui Xu, Yanbo Chen, Qi Chen, Huan Xu, Yanxia Wang, Jiao Yu, Juan Zhou, Zhong Wang, Bing Xu, DNMT1 regulates IL-6- and TGF-β1-induced epithelial mesenchymal transition in prostate epithelial cells , European Journal of Histochemistry: Vol. 61 No. 2 (2017)
- G. Lattanzi, S. Marmiroli, A. Facchini, N.M. Maraldi, Nuclear damages and oxidative stress: new perspectives for laminopathies , European Journal of Histochemistry: Vol. 56 No. 4 (2012)
- E. Negrato, M. Vascellari, F. Capolongo, G. Binato, L. Da Dalt, M. Boscolo Papo, G. Gioacchini, O. Carnevali, D. Bertotto, G. Radaelli, F. Pascoli, Expression of 8-OHdG in Zosterisessor ophiocephalus from the Venetian lagoon, Italy , European Journal of Histochemistry: Vol. 57 No. 1 (2013)
<< < 1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.
