https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 198
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- H. Zhang, Y. Wang, J. Zhang, L. Wang, Q. Li, X. Sheng, Y. Han, Z. Yuan, Q. Weng, Testicular expression of NGF, TrkA and p75 during seasonal spermatogenesis of the wild ground squirrel (Citellus dauricus Brandt) , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- A. Arcucci, M.R. Ruocco, F. Albano, G. Granato, V. Romano, G. Corso, C. Bancone, E. De Vendittis, A. Della Corte, S. Montagnani, Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve , European Journal of Histochemistry: Vol. 58 No. 3 (2014)
- R. Song, W. Xu, Y. Chen, Z. Li, Y. Zeng, Y. Fu, The expression of Sirtuins 1 and 4 in peripheral blood leukocytes from patients with type 2 diabetes , European Journal of Histochemistry: Vol. 55 No. 1 (2011)
- Jinbi Xie, Yong Ning, Lihang Zhang, Yuan Lin, Runsheng Guo, Shanjuan Wang, Overexpression of hsa_circ_0006470 inhibits the malignant behavior of gastric cancer cells via regulation of miR-1234/TP53I11 axis , European Journal of Histochemistry: Vol. 66 No. 4 (2022)
- Guangbao He, Yibo He, Hongwei Ni, Kai Wang, Yijun Zhu, Yang Bao, Dexmedetomidine attenuates neuroinflammation and microglia activation in LPS-stimulated BV2 microglia cells through targeting circ-Shank3/miR-140-3p/TLR4 axis , European Journal of Histochemistry: Vol. 67 No. 3 (2023)
- A. Di Vito, E. Scali, G. Ferraro, C. Mignogna, I. Presta, C. Camastra, C. Palmieri, G. Donato, T. Barni, Elastofibroma dorsi: a histochemical and immunohistochemical study , European Journal of Histochemistry: Vol. 59 No. 1 (2015)
- J. Feng, S. Ge, L. Zhang, H. Che, C. Liang, Aortic dissection is associated with reduced polycystin-1 expression, an abnormality that leads to increased ERK phosphorylation in vascular smooth muscle cells , European Journal of Histochemistry: Vol. 60 No. 4 (2016)
- L. Ragionieri, M. Botti, F. Gazza, C. Sorteni, R. Chiocchetti, P. Clavenzani, L. Bo, R. Panu, Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component , European Journal of Histochemistry: Vol. 57 No. 2 (2013)
- G. Goteri, E. Altobelli, G. Tossetta, A. Zizzi, C. Avellini, C. Licini, T. Lorenzi, M. Castellucci, A. Ciavattini, D. Marzioni, High temperature requirement A1, transforming growth factor beta1, phosphoSmad2 and Ki67 in eutopic and ectopic endometrium of women with endometriosis , European Journal of Histochemistry: Vol. 59 No. 4 (2015)
- Cristian Turato, Mariapia Vairetti, Marta Cagna, Alessandra Biasiolo, Andrea Ferrigno, Santina Quarta, Mariagrazia Ruvoletto, Silvia De Siervi, Patrizia Pontisso, Laura Giuseppina Di Pasqua, SerpinB3 administration protects liver against ischemia-reperfusion injury , European Journal of Histochemistry: Vol. 66 No. 4 (2022)
<< < 23 24 25 26 27 28 29 30 31 32 > >>
You may also start an advanced similarity search for this article.
