https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 197
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- E. Ayarza, M. González, F. López, R. Fernández-Donoso, J. Page, S. Berrios, Alterations in chromosomal synapses and DNA repair in apoptotic spermatocytes of Mus m. domesticus , European Journal of Histochemistry: Vol. 60 No. 2 (2016)
- Yuuki Maeda, Yoko Miwa, Iwao Sato, Expression of CGRP, vasculogenesis and osteogenesis associated mRNAs in the developing mouse mandible and tibia , European Journal of Histochemistry: Vol. 61 No. 1 (2017)
- F.M. Gomes, I.B. Ramos, C. Wendt, W. Girard-Dias, W. De Souza, E.A. Machado, K. Miranda, New insights into the in situ microscopic visualization and quantification of inorganic polyphosphate stores by 4',6-diamidino-2-phenylindole (DAPI)-staining , European Journal of Histochemistry: Vol. 57 No. 4 (2013)
- M. Ferreira Júnior, S.A. Batista, P.V.T. Vidigal, A.A.C. Cordeiro, F.M.S. Oliveira, L.O. Prata, A.E.T. Diniz, C.M. Barral, R.C. Barbuto, A.D. Gomes, I.D. Araújo, D.M.M. Queiroz, M.V. Caliari, Infection with CagA-positive Helicobacter pylori strain containing three EPIYA C phosphorylation sites is associated with more severe gastric lesions in experimentally infected Mongolian gerbils (Meriones unguiculatus) , European Journal of Histochemistry: Vol. 59 No. 2 (2015)
- E. Carabajal, N. Massari, M. Croci, D. J. Martinel Lamas, J. P. Prestifilippo, R. M. Bergoc, E. S. Rivera, V. A. Medina, Radioprotective potential of histamine on rat small intestine and uterus , European Journal of Histochemistry: Vol. 56 No. 4 (2012)
- Giuliano Mazzini, The Feulgen reaction: from pink-magenta to rainbow fluorescent at the Maffo Vialli’s School of Histochemistry , European Journal of Histochemistry: Vol. 68 No. 1 (2024): 1954-2024: 70 Years of Histochemical Research
- M. Lehmann, F. Martin, K. Mannigel, K. Kaltschmidt, U. Sack, U. Anderer, Three-dimensional scaffold-free fusion culture: the way to enhance chondrogenesis of in vitro propagated human articular chondrocytes , European Journal of Histochemistry: Vol. 57 No. 4 (2013)
- S. Shibata, Y. Sakamoto, O. Baba, C. Qin, G. Murakami, B.H. Cho, An immunohistochemical study of matrix proteins in the craniofacial cartilage in midterm human fetuses , European Journal of Histochemistry: Vol. 57 No. 4 (2013)
- A.R.M. Sabbatini, L. Mattii, B. Battolla, E. Polizzi, D. Martini, M. Ranieri-Raggi, A.J.G. Moir, A. Raggi, Evidence that muscle cells do not express the histidine-rich glycoprotein associated with AMP deaminase but can internalise the plasma protein , European Journal of Histochemistry: Vol. 55 No. 1 (2011)
- V. Giansanti, G. Santamaria, A. Torriglia, F. Aredia, A.I. Scovassi, G. Bottiroli, A.C. Croce, Fluorescence properties of the Na+/H+exchanger inhibitor HMA (5-(N,N-hexamethylene)amiloride) are modulated by intracellular pH , European Journal of Histochemistry: Vol. 56 No. 1 (2012)
<< < 1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.
