https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 198
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- G. Natale, E. Pompili, F. Biagioni, S. Paparelli, P. Lenzi, F. Fornai, Histochemical approaches to assess cell-to-cell transmission of misfolded proteins in neurodegenerative diseases , European Journal of Histochemistry: Vol. 57 No. 1 (2013)
- Viktória Dénes, Kármen Kovacs, Ákos Lukáts, Adrienn Mester, Gergely Berta, Arnold Szabó, Robert Gabriel, Secreted key regulators (Fgf1, Bmp4, Gdf3) are expressed by PAC1-immunopositive retinal ganglion cells in the postnatal rat retina , European Journal of Histochemistry: Vol. 66 No. 2 (2022)
- S. Strobel, J.A. Encarnação, N.I. Becker, T.E. Trenczek, Histological and histochemical analysis of the gastrointestinal tract of the common pipistrelle bat (Pipistrellus pipistrellus) , European Journal of Histochemistry: Vol. 59 No. 2 (2015)
- Yang Liu, Weichun Guo, Shuo Fang, Bin He, Xiaohai Li, Li Fan, miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin , European Journal of Histochemistry: Vol. 65 No. 4 (2021)
- Zeqi Tang, Xiaojie Yuan, Yuming Bai, Yiming Guo, Haolin Zhang, Yingying Han, Zhengrong Yuan, Qiang Weng, Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (Ondatra zibethicus) , European Journal of Histochemistry: Vol. 66 No. 2 (2022)
- AM Cianciulli, R Bovani, C Leonardo, F Iori, AM Coletta, R Marzano, A Antenucci, GM Gandolfo, C Laurenti, DNA aberrations in urinary bladder cancer detected by flow cytometry and fish: prognostic implications , European Journal of Histochemistry: Vol. 45 No. 1 (2001)
- Chaojie He, Yi Yu, Feifan Wang, Wudi Li, Hui Ni, Meixiang Xiang, Pretreatment with interleukin-15 attenuates inflammation and apoptosis by inhibiting NF-κB signaling in sepsis-induced myocardial dysfunction , European Journal of Histochemistry: Vol. 68 No. 2 (2024)
- D. Stępiński, Immunofluorescent localization of ubiquitin and proteasomes in nucleolar vacuoles of soybean root meristematic cells , European Journal of Histochemistry: Vol. 56 No. 2 (2012)
- Jinshuang Li, Dawei Xu, Ce Shi, Chunqi Cheng, Ziheng Xu, Xingjuan Gao, Yong Cheng, Alarin regulates RyR2 and SERCA2 to improve cardiac function in heart failure with preserved ejection fraction , European Journal of Histochemistry: Vol. 68 No. 4 (2024)
- Xiaoyan Chu, Jun Lou, Yun Yi, Linlin Zhong, Ouping Huang, Knockdown of ARHGAP30 inhibits ovarian cancer cell proliferation, migration, and invasiveness by suppressing the PI3K/AKT/mTOR signaling pathway , European Journal of Histochemistry: Vol. 67 No. 2 (2023)
<< < 34 35 36 37 38 39 40 41 42 43 > >>
You may also start an advanced similarity search for this article.
