https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 198
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- R. M. Ruggeri, E. Vitarelli, G. Barresi, F. Trimarchi, S. Benvenga, M. Trovato, The tyrosine kinase receptor c-met, its cognate ligand HGF and the tyrosine kinase receptor trasducers STAT3, PI3K and RHO in thyroid nodules associated with Hashimoto's thyroiditis: an immunohistochemical characterization , European Journal of Histochemistry: Vol. 54 No. 2 (2010)
- CarloAlberto Redi, Light microscopy - Methods and protocols , European Journal of Histochemistry: Vol. 55 No. 4 (2011)
- S. Nemolato, J. Ekstrom, T. Cabras, C. Gerosa, D. Fanni, E. Di Felice, A. Locci, I. Messana, M. Castagnola, G. Faa, Immunoreactivity for thymosin beta 4 and thymosin beta 10 in the adult rat oro-gastro-intestinal tract , European Journal of Histochemistry: Vol. 57 No. 2 (2013)
- M. Salemi, A. E. Calogero, G. Zaccarello, R. Castiglione, A. Cosentino, C. Campagna, E. Vicari, G. Rappazzo, Expression of SPANX proteins in normal prostatic tissue and in prostate cancer , European Journal of Histochemistry: Vol. 54 No. 3 (2010)
- Brigitta Bonaldo, Antonino Casile, Martina Bettarelli, Stefano Gotti, GianCarlo Panzica, Marilena Marraudino, Effects of chronic exposure to bisphenol A in adult female mice on social behavior, vasopressin system, and estrogen membrane receptor (GPER1) , European Journal of Histochemistry: Vol. 65 No. s1 (2021): Special Collection on Advances in Neuromorphology in Health and Disease
- I.M.S. Paulsen, H. Dimke, S. Frische, A single simple procedure for dewaxing, hydration and heat-induced epitope retrieval (HIER) for immunohistochemistry in formalin fixed paraffin-embedded tissue , European Journal of Histochemistry: Vol. 59 No. 4 (2015)
- Flavia Carton, Laura Calderan, Manuela Malatesta, Incubation under fluid dynamic conditions markedly improves the structural preservation in vitro of explanted skeletal muscles , European Journal of Histochemistry: Vol. 61 No. 4 (2017)
- Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta, 1954-2024: 70 years of histochemical research with the European Journal of Histochemistry , European Journal of Histochemistry: Vol. 68 No. 1 (2024): 1954-2024: 70 Years of Histochemical Research
- Tao Wu, Shikui Wu, Hui Gao, Haolei Liu, Jun Feng, Ge Yin, Astragaloside IV augments anti-PD-1 therapy to suppress tumor growth in lung cancer by remodeling the tumor microenvironment , European Journal of Histochemistry: Vol. 68 No. 4 (2024)
- Eetmad A. Arafat, Eman M.I. Youssef, Hanaa A. Khalaf, The possible alleviating effect of garlic supplement on the neural retina in a rat model of hypercholesterolemia: a histological and immunohistochemical study , European Journal of Histochemistry: Vol. 65 No. 4 (2021)
<< < 40 41 42 43 44 45 46 47 48 49 > >>
You may also start an advanced similarity search for this article.
