https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 198
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- A Gigante, S Manzotti, C Bevilacqua, M Orciani, R Di Primio, M Mattioli-Belmonte, Adult mesenchymal stem cells for bone and cartilage engineering: effect of scaffold materials , European Journal of Histochemistry: Vol. 52 No. 3 (2008)
- Sheila Veronese, Ezio Costa, Antonella Portuese, Riccardo Ossanna, Andrea Sbarbati, Histological analysis of the dermal and hypodermal layers of the face and correlation with high-frequency 24 MHz ultrasonography and elastosonography , European Journal of Histochemistry: Vol. 68 No. 2 (2024)
- R Acevedo, A Cuadrado, C De la Torre, S Moreno Díaz de la Espina, Behaviour of ribosomal genes and nucleolar domains during activation in sugarcane (Saccharum officinarum L.) root primordia: from the unsoaked quiescent state to the steady state of proliferation , European Journal of Histochemistry: Vol. 46 No. 2 (2002)
- G Mazzini, C Ferrari, E Erba, Dual excitation multi-fluorescence flow cytometry for detailed analyses of viability and apoptotic cell transition , European Journal of Histochemistry: Vol. 47 No. 4 (2003)
- RG Steinbeck, Dysplasia in view of the cell cycle , European Journal of Histochemistry: Vol. 48 No. 3 (2004)
- Zhi-bao Liu, Jing-hua Zhang, Jing-hua Gao, Jian Shi, Effects of the lncRNA ENST00000623984 on colon cancer and the biological characteristics of colon cancer cells , European Journal of Histochemistry: Vol. 65 No. 3 (2021)
- D. Curzi, S. Salucci, M. Marini, F. Esposito, L. Agnello, A. Veicsteinas, S. Burattini, E. Falcieri, How physical exercise changes rat myotendinous junctions: an ultrastructural study , European Journal of Histochemistry: Vol. 56 No. 2 (2012)
- K Smetana, H Klamová, D Mikulenková, M Pluskalová, Z Hrkal, On the nucleolar size and density in human early granulocytic progenitors, myeloblasts , European Journal of Histochemistry: Vol. 50 No. 2 (2006)
- C. Soldani, M.G. Bottone, M. Biggiogera, C. Alpini, A.I. Scovassi, T. Martin, C. Pellicciari, Nuclear localization of phosphorylated c-Myc protein in human tumor cells. , European Journal of Histochemistry: Vol. 46 No. 4 (2002)
- Arianna Casini, Rosa Vaccaro, Mattia Toni, Carla Cioni, Distribution of choline acetyltransferase (ChAT) immunoreactivity in the brain of the teleost Cyprinus carpio , European Journal of Histochemistry: Vol. 62 No. 3 (2018)
<< < 52 53 54 55 56 57 58 59 60 61 > >>
You may also start an advanced similarity search for this article.
