https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 197
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- C. Pellicciari, Histochemistry through the years, browsing a long-established journal: novelties in traditional subjects , European Journal of Histochemistry: Vol. 54 No. 4 (2010)
- V. Pibiri, A. Ravarino, C. Gerosa, M.C. Pintus, V. Fanos, G. Faa, Stem/progenitor cells in the developing human cerebellum: an immunohistochemical study , European Journal of Histochemistry: Vol. 60 No. 3 (2016)
- M. Costanzo, F. Carton, A. Marengo, G. Berlier, B. Stella, S. Arpicco, M. Malatesta, Fluorescence and electron microscopy to visualize the intracellular fate of nanoparticles for drug delivery , European Journal of Histochemistry: Vol. 60 No. 2 (2016)
- M.G. Bottone, G. Santin, C. Soldani, P. Veneroni, A.I. Scovassi, C. Alpini, Intracellular distribution of Tankyrases as detected by multicolor immunofluorescence techniques , European Journal of Histochemistry: Vol. 56 No. 1 (2012)
- Marco Biggiogera, The Feulgen reaction at the electron microscopy level , European Journal of Histochemistry: Vol. 68 No. 1 (2024): 1954-2024: 70 Years of Histochemical Research
- G. Musumeci, C. Loreto, M.L. Carnazza, F. Coppolino, V. Cardile, R. Leonardi, Lubricin is expressed in chondrocytes derived from osteoarthritic cartilage encapsulated in poly(ethylene glycol) diacrylate scaffold , European Journal of Histochemistry: Vol. 55 No. 3 (2011)
- D. Nowak, A. J. Mazur, A. Popow-Woźniak, A. Radwańska, H. G. Mannherz, M. Malicka-Błaszkiewicz, Subcellular distribution and expression of cofilin and ezrin in human colon adenocarcinoma cell lines with different metastatic potential , European Journal of Histochemistry: Vol. 54 No. 2 (2010)
- C. Loreto, C. Galanti, G. Musumeci, M. C. Rusu, R. Leonardi, Immunohistochemical analysis of matrix metalloproteinase-13 in human caries dentin , European Journal of Histochemistry: Vol. 58 No. 1 (2014)
- F. Aredia, M. Malatesta, P. Veneroni, M.G. Bottone, Analysis of ERK3 intracellular localization: dynamic distribution during mitosis and apoptosis , European Journal of Histochemistry: Vol. 59 No. 4 (2015)
- Mariailaria Verderame, Ermelinda Limatola, Rosaria Scudiero, Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposure , European Journal of Histochemistry: Vol. 61 No. 2 (2017)
<< < 1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.
