https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 198
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- N. Tolosa de Talamoni, A. Pérez, R. Riis, C. Smith, M. L. Norman, R. H. Wasserman, Comparative immunolocalization of the plasma membrane calcium pump and calbindin D28K in chicken retina during embryonic development , European Journal of Histochemistry: Vol. 46 No. 4 (2002)
- Manuela Monti, Aging and Health - A system biology perspective , European Journal of Histochemistry: Vol. 59 No. 1 (2015)
- Claudio Pigoli, Lucia Rita Gibelli, Mario Caniatti, Luca Moretti, Giuseppe Sironi, Chiara Giudice, Bleaching melanin in formalin-fixed and paraffin-embedded melanoma specimens using visible light: a pilot study , European Journal of Histochemistry: Vol. 63 No. 4 (2019)
- G Natale, G Lazzeri, C Blandizzi, M Ferrucci, M Del Tacca, Differential distribution of transforming growth factor-a immunohistochemistry within whole gastric mucosa in rats , European Journal of Histochemistry: Vol. 47 No. 4 (2003)
- Jinjiao Li, Panshi Zhang, Yun Xia, Aberrant expression of CCDC69 in breast cancer and its clinicopathologic significance , European Journal of Histochemistry: Vol. 65 No. 1 (2021)
- S-K Ku, H-S Lee, The distribution and frequency of endocrine cells in the splenic lobe of grass lizard (Takydromus wolteri): An immunohistochemical study , European Journal of Histochemistry: Vol. 48 No. 4 (2004)
- M Malatesta, B Baldelli, S Battistelli, C Tiberi, E Manuali, M Biggiogera, Reversibility of hepatocyte nuclear modifications in mice fed on genetically modified soybean , European Journal of Histochemistry: Vol. 49 No. 3 (2005)
- S Ganassi, D Tagliazucchi, L Mola, Occurrence of nitric oxide synthase in Megoura viciae Buckton(Homoptera, Aphididae): an histochemical and immunohistochemical localisation , European Journal of Histochemistry: Vol. 49 No. 4 (2005)
- R Toni, M Vitale, The role of flow cytometry in the study of cell growth in the rat anterior pituitary gland , European Journal of Histochemistry: Vol. 44 No. 4 (2000)
- H Ishida, M Kumakiri, K Ueda, LM Lao, M Yanagihara, K Asamoto, Y Imamura, S Noriki, M Fukuda, Comparative histochemical study of Bowen’s disease and actinic keratosis: preserved normal basal cells in Bowen’s disease , European Journal of Histochemistry: Vol. 45 No. 2 (2001)
<< < 70 71 72 73 74 75 76 77 78 79 > >>
You may also start an advanced similarity search for this article.
