https://doi.org/10.4081/ejh.2017.2773
Expression of the HPV18/E6 oncoprotein induces DNA damage
Accepted: 30 April 2017
HTML: 197
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- M Malatesta, F Perdoni, S Muller, C Zancanaro, C Pellicciari, Nuclei of aged myofibres undergo structural and functional changes suggesting impairment in RNA processing , European Journal of Histochemistry: Vol. 53 No. 2 (2009)
- L. Mola, D. Sassi, B. Cuoghi, The supramedullary cells of the teleost Coris julis (L.): a noradrenergic neuronal system , European Journal of Histochemistry: Vol. 46 No. 4 (2002)
- Carlo Alberto Redi, 3D cell culture - Methods and protocols , European Journal of Histochemistry: Vol. 55 No. 2 (2011)
- Manuela Monti, Mouse development - From oocyte to stem cells , European Journal of Histochemistry: Vol. 58 No. 1 (2014)
- S Matsubara, D Matsubara, T Ishibashi, T Takizawa, Demonstration of glucose-6-phosphate dehydrogenase in rat Kupffer cells by a newly-developed ultrastructural enzyme-cytochemistry , European Journal of Histochemistry: Vol. 47 No. 2 (2003)
- Rosa Manca, Chester Glomski , Alessandra Pica, Hematopoietic stem cells debut in embryonic lymphomyeloid tissues of elasmobranchs , European Journal of Histochemistry: Vol. 63 No. 3 (2019)
- F Parillo, C Dall’Aglio, A Verini Supplizi, P Ceccarelli, AM Gargiulo, Immunogold study on lectin binding in the porcine zona pellucida and granulosa cells , European Journal of Histochemistry: Vol. 47 No. 4 (2003)
- T Yasui, A Tsukise, W Meyer, Histochemical analysis of glycoconjugates in the eccrine glands of the raccoon digital pads , European Journal of Histochemistry: Vol. 48 No. 4 (2004)
- C Domeneghini, G Radaelli, S Arrighi, F Mascarello, A Veggetti, Neurotransmitters and putative neuromodulators in the gut of Anguilla anguilla (L.). Localizations in the enteric nervous and endocrine systems , European Journal of Histochemistry: Vol. 44 No. 3 (2000)
- H. Zhang, X. Guo, S. Zhong, T. Ge, S. Peng, P. Yu, Z. Zhou, Heterogeneous vesicles in mucous epithelial cells of posterior esophagus of Chinese giant salamander (Andrias davidianus) , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
<< < 71 72 73 74 75 76 77 78 79 > >>
You may also start an advanced similarity search for this article.
