https://doi.org/10.4081/ejh.2017.2773

Expression of the HPV18/E6 oncoprotein induces DNA damage

Vol. 61 No. 2 (2017)
Submitted: 17 January 2017
Accepted: 30 April 2017
Published: 15 June 2017
DNA damage, DNA breakage detection-fluorescence in situ hybridization, DBD-FISH, human papillomavirus, HPV, E6 oncogene, alkaline-labile sites.
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Authors

Elva I. Cortés Gutiérrez
elvacortes@cibinmty.net
Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica del Noreste, Mexico.
Catalina Garcí­a-Vielma
Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica del Noreste, Mexico.
Adriana Aguilar-Lemarroy
Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Occidente, Mexico.
Veronica Vallejo-Ruí­z
Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Occidente, Mexico.
Patricia Piña-Sánchez
Instituto Mexicano del Seguro Social, Unidad de Investigación Médica en Enfermedades Oncológicas, Mexico.
Pablo Zapata-Benavides
Universidad Autónoma de Nuevo León, Faculty of Biology, Mexico.
Jaime Gosalvez
Universidad Autónoma de Madrid, Department of Biology, Spain.

This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.

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Supporting Agencies

Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).

How to Cite

Cortés Gutiérrez, E. I., Garcí­a-Vielma, C., Aguilar-Lemarroy, A., Vallejo-Ruí­z, V., Piña-Sánchez, P., Zapata-Benavides, P., & Gosalvez, J. (2017). Expression of the HPV18/E6 oncoprotein induces DNA damage. European Journal of Histochemistry, 61(2). https://doi.org/10.4081/ejh.2017.2773

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