https://doi.org/10.4081/ejh.2017.2773
0
0
0
0
Smart Citations
0
0
0
0
Citing PublicationsSupportingMentioningContrasting
View Citations

See how this article has been cited at scite.ai

scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

Expression of the HPV18/E6 oncoprotein induces DNA damage

Authors

Elva I. Cortés Gutiérrez
elvacortes@cibinmty.net
Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica del Noreste, Mexico.
Catalina Garcí­a-Vielma
Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica del Noreste, Mexico.
Adriana Aguilar-Lemarroy
Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Occidente, Mexico.
Veronica Vallejo-Ruí­z
Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Occidente, Mexico.
Patricia Piña-Sánchez
Instituto Mexicano del Seguro Social, Unidad de Investigación Médica en Enfermedades Oncológicas, Mexico.
Pablo Zapata-Benavides
Universidad Autónoma de Nuevo León, Faculty of Biology, Mexico.
Jaime Gosalvez
Universidad Autónoma de Madrid, Department of Biology, Spain.

This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.

Altmetrics

Plum Print visual indicator of research metrics
  • Citations
    • Citation Indexes: 10
  • Captures
    • Readers: 18
see details

Downloads

Citations

Crossref
Scopus
Google Scholar
Europe PMC

Supporting Agencies

Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).

How to Cite



Expression of the HPV18/E6 oncoprotein induces DNA damage. (2017). European Journal of Histochemistry, 61(2). https://doi.org/10.4081/ejh.2017.2773

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.