https://doi.org/10.4081/ejh.2017.2783
Characterization of the role of RILP in cell migration
Accepted: 18 May 2017
Supplementary: 205
Movie 1: 263
Movie 2: 309
HTML: 94
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Rab-interacting lysosomal protein (RILP) is a regulator of late stages of endocytosis. Recent work proved that depletion of RILP promotes migration of breast cancer cells in wound healing assay, whereas its overexpression influences re-arrangements of actin cytoskeleton. Here, we further characterized the role of RILP in cell migration by analyzing several aspects of this process. We showed that RILP is fundamental also for migration of lung cancer cells regulating cell velocity. RILP silencing did not affect Golgi apparatus nor microtubules reorientation during migration. However, both RILP over-expression and expression of its mutated form, RILP-C33, impair cell adhesion and spreading. In conclusion, our results demonstrate that RILP has important regulatory roles in cell motility affecting migration velocity but also in cell adhesion and cell spreading.
Supporting Agencies
AIRC (Grant IG19068 to CB), Telethon Italy (Grant GGP16037 to CB), Norwegian Cancer Society (Grants 5760850 to CP and 4604944 to OB), Research Council of Norway (grants 239903 to CP, 230779 to OB, and Centre of Excellence funding project 179573)How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- A. Arcucci, M.R. Ruocco, F. Albano, G. Granato, V. Romano, G. Corso, C. Bancone, E. De Vendittis, A. Della Corte, S. Montagnani, Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve , European Journal of Histochemistry: Vol. 58 No. 3 (2014)
- V. Cenni, C. Capanni, M. Columbaro, M. Ortolani, M.R. D'Apice, G. Novelli, M. Fini, S. Marmiroli, E. Scarano, N.M. Maraldi, S. Squarzoni, S. Prencipe, G. Lattanzi, Autophagic degradation of farnesylated prelamin A as a therapeutic approach to lamin-linked progeria , European Journal of Histochemistry: Vol. 55 No. 4 (2011)
- A. Amaroli, M.G. Chessa, Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa) , European Journal of Histochemistry: Vol. 56 No. 4 (2012)
- G. Laguna Hernández, A.E. Brechú-Franco, I. De la Cruz-Chacón, A.R. González-Esquinca, Histochemical detection of acetogenins and storage molecules in the endosperm of Annona macroprophyllata Donn Sm. seeds , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- F. Piras, M.T. Ionta, S. Lai, M.T. Perra, F. Atzori, L. Minerba, V. Pusceddu, C. Maxia, D. Murtas, P. Demurtas, B. Massidda, P. Sirigu, Nestin expression associates with poor prognosis and triple negative phenotype in locally advanced (T4) breast cancer , European Journal of Histochemistry: Vol. 55 No. 4 (2011)
- S. Salucci, P. Ambrogini, D. Lattanzi, M. Betti, P. Gobbi, C. Galati, F. Galli, R. Cuppini, A. Minelli, Maternal dietary loads of alpha-tocopherol increase synapse density and glial synaptic coverage in the hippocampus of adult offspring , European Journal of Histochemistry: Vol. 58 No. 2 (2014)
- Mengling Li, Yan Zhao, Jiayi Zhang, Wang Jiang, Siyuan Peng, Jinyue Hu, Yueming Shen, Deubiquitinase USP14 is upregulated in Crohn's disease and inhibits the NOD2 pathway mediated inflammatory response in vitro , European Journal of Histochemistry: Vol. 68 No. 3 (2024)
- C. Loreto, R. Leonardi, G. Musumeci, G. Pannone, S. Castorina, An ex vivo study on immunohistochemical localization of MMP-7 and MMP-9 in temporomandibular joint discs with internal derangement , European Journal of Histochemistry: Vol. 57 No. 2 (2013)
- Bita Moudi, Zahra Heidari, Hamidreza Mahmoudzadeh-Sagheb, Seyed-Moayed Alavian, Kamran B. Lankarani, Parisa Farrokh, Jens Randel Nyengaard, Concomitant use of heat-shock protein 70, glutamine synthetase and glypican-3 is useful in diagnosis of HBV-related hepatocellular carcinoma with higher specificity and sensitivity , European Journal of Histochemistry: Vol. 62 No. 1 (2018)
- D.E. Korzhevskii, E.G. Sukhorukova, O.V. Kirik, I.P. Grigorev, Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
<< < 33 34 35 36 37 38 39 40 41 42 > >>
You may also start an advanced similarity search for this article.
