Protective effect of different antioxidant agents in UVB-irradiated keratinocytes

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Sara Salucci
Sabrina Burattini
Francesca Buontempo
Alberto Maria Martelli
Elisabetta Falcieri
Michela Battistelli *
(*) Corresponding Author:
Michela Battistelli | michela.battistelli@uniurb.it

Abstract

Skin cells can respond to UVB-induced damage either by tolerating it, or restoring it through antioxidant activation and DNA repair mechanisms or, ultimately, undergoing programmed cell death, when damage is massive. Nutritional factors, in particular, food antioxidants, have attracted much interest because of their potential use in new preventive, protective, and therapeutic strategies for chronic degenerative diseases, including skin inflammation and cancer. Some polyphenols, present in virgin olive oil, well tolerated by organism after oral administration, show a variety of pharmacological and clinical benefits such as anti-oxidant, anti-cancer, anti-inflammatory, and neuro-protective activities. Here, the protective effects of antioxidant compounds against UV-induced apoptosis have been described in HaCat cell line. Human keratinocytes were pre-treated with antioxidants before UVB exposure and their effects have been evaluated by means of ultrastructural analyses. After UVB radiation, a known cell death trigger, typical apoptotic features, absent in control condition and in antioxidant alone-treated cells, appear. An evident numerical decrease of ultrastructural apoptotic patterns and TUNEL positive nuclei can be observed when natural antioxidants were supplied before cell death induction. These data have been confirmed by molecular investigation of caspase activity. In conclusion, this paper highlights antioxidant compound ability to prevent apoptotic cell death in human keratinocytes exposed to UVB, suggesting, for these molecules, a potential role in preventing skin damage. 


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Author Biographies

Sara Salucci, University of Urbino “Carlo Bo”

Department of Biomolecular Sciences

Sabrina Burattini, University of Urbino “Carlo Bo”

Department of Biomolecular Sciences

Francesca Buontempo, University of Bologna

Department of Biomedical and Neuromotor Sciences

Alberto Maria Martelli, University of Bologna

Department of Biomedical and Neuromotor Sciences

Elisabetta Falcieri, University of Urbino “Carlo Bo”

Department of Biomolecular Sciences

Michela Battistelli, University of Urbino “Carlo Bo”

Department of Biomolecular Sciences