Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium

  • Andrea Ferrigno University of Pavia, Department of Internal Medicine and Therapeutics, Italy.
  • Laura G. Di Pasqua University of Pavia, Department of Internal Medicine and Therapeutics, Italy.
  • Clarissa Berardo University of Pavia, Department of Internal Medicine and Therapeutics, Italy.
  • Veronica Siciliano University of Pavia, Department of Internal Medicine and Therapeutics, Italy.
  • Plinio Richelmi University of Pavia, Department of Internal Medicine and Therapeutics, Italy.
  • Mariapia Vairetti | mariapia.vairetti@unipv.it University of Pavia, Department of Internal Medicine and Therapeutics, Italy. http://orcid.org/0000-0002-2464-6127

Abstract

The role of Na+ in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N2-saturated buffer containing standard (143 mM) or low (25 mM) Na+ levels. Trypan blue uptake was used to detect non-viable cells. Under hypoxia with standard-Na+, trypan blue uptake began at the border between pericentral areas and periportal regions and increased in the latter zone; using a low-Na+ buffer, no trypan blue zonation occurred but a homogenous distribution of dye was found associated with sinusoidal endothelial cell (SEC) staining. A decrease in hyaluronic acid (HA) uptake, index of SEC damage, was observed using a low-Na+ buffer. A time dependent injury was confirmed by an increase in LDH and TBARS levels with standard-Na+ buffer. Using low-Na+ buffer, SEC susceptibility appears elevated under hypoxia and hepatocytes was protected, in an oxygen independent manner.

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Published
2017-05-30
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Section
Brief Reports
Keywords:
Liver, hypoxia, Na , hepatocytes, sinusoidal endothelial cells.
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How to Cite
Ferrigno, A., Di Pasqua, L. G., Berardo, C., Siciliano, V., Richelmi, P., & Vairetti, M. (2017). Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium. European Journal of Histochemistry, 61(2). https://doi.org/10.4081/ejh.2017.2798