Ultrastructure and immunohistochemical characterization of proteins concerned with the secretory machinery in goat ceruminous glands

  • Tadashi Yasui | yasui.tadashi@nihon-u.ac.jp Nihon University, Department of Veterinary Anatomy, College of Bioresource Sciences, Japan.
  • Hiroshi Gomi Nihon University, Department of Veterinary Anatomy, College of Bioresource Sciences, Japan.
  • Taishi Kitahara Nihon University, Department of Veterinary Anatomy, College of Bioresource Sciences, Japan.
  • Azuma Tsukise Nihon University, Department of Veterinary Anatomy, College of Bioresource Sciences, Japan.

Abstract

The expression of soluble N-ethyl-maleimide sensitive fusion attachment protein receptor (SNARE) proteins in apocrine glands has not been fully elucidated. In addition to performing ultrastructural observation of the ceruminous glands in goats, our study focuses on the demonstration of β-defensins, SNARE proteins and Rab3D in these glands with the use of immunohistochemical methods. The secretory cells were equipped with two types of vesicles, Golgi apparatus and abundant rough endoplasmic reticulum (ER). Additionally, in some of them, the characteristic concentric structures composed of rough ER were observed in their circum- and infranuclear parts. The expression of phosphorylated inositol requiring enzyme 1a was also detected. These findings may indicate their ability to produce numerous secretory proteins and the maintenance of homeostasis in the glandular cells. Furthermore, β-defensins were demonstrated as products of the ceruminous glands. The present investigation also revealed the presence of SNARE proteins and Rab3D. It is suggested that these proteins are concerned with the secretory machinery of this gland type.

Downloads

Download data is not yet available.
Published
2017-08-08
Section
Original Papers
Keywords:
ceruminous glands, goat, secretory machinery, SNARE proteins, immunohistochemistry, ER stress,
Statistics
Abstract views: 580

PDF: 170
HTML: 134
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Yasui, T., Gomi, H., Kitahara, T., & Tsukise, A. (2017). Ultrastructure and immunohistochemical characterization of proteins concerned with the secretory machinery in goat ceruminous glands. European Journal of Histochemistry, 61(3). https://doi.org/10.4081/ejh.2017.2828